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Rapalink-1

Name: Rapalink-1
Brand: TargetMol
SKU: TP2288
Rating: 4.5 (1 reviews)

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Catalog No. TP2288Cas No. 1887095-82-0

Rapalink-1 is a brain-permeable mTOR inhibitor that inhibits oxidative stress-induced DNA damage and senescence in ethanol-exposed endothelial cells, and inhibits the mTORC1-4E-BP1 pathway in mice.Rapalink-1 is used for the study of prostate cancer due to its anticancer activity.

Rapalink-1

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Catalog No. TP2288Cas No. 1887095-82-0

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 mg	$1,200	7-10 days	7-10 days
10 mg	$2,235	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	Rapalink-1 is a brain-permeable mTOR inhibitor that inhibits oxidative stress-induced DNA damage and senescence in ethanol-exposed endothelial cells, and inhibits the mTORC1-4E-BP1 pathway in mice.Rapalink-1 is used for the study of prostate cancer due to its anticancer activity.
In vitro	METHODS: U87MG cells were lysed with RapaLink-1 (0.39, 0.78, 1.56, 3.13, 6.25, 12.5 nM, 3 hours) and analyzed by western blot as indicated. RESULTS: RapaLink-1 selectively inhibited p-RPS6S235/236 and p-4EBP1T37/46 at doses as low as 1.56 nM. mTORC2 targeting p-AKTS473, p-SGK1S78, and p-NDRG1T346 and p-AKTS473 targeting p-GSK3βS9 were inhibited only at high doses. [1]
In vivo	METHODS: BALB/cNU/NU mice were treated with RapaLink-1 (0.4 mg/kg or 4 mg/kg, ip, 15 min) and injected intraperitoneally with 250 mU insulin or saline for 15 min. Mice were sacrificed, and skeletal muscle, liver, and brain were harvested, lysed, and analyzed by Western blotting as indicated. RESULTS: RapaLink-1 was able to inhibit the expression of p-RPS6S235/236 and p-4EBP1T37/46 in the brain in a concentration-dependent manner, but did not inhibit the expression of the mTORC2 substrate p-AKTS473. [1]

Chemical Properties

Molecular Weight	1784.14
Formula	C₉₁H₁₃₈N₁₂O₂₄
Cas No.	1887095-82-0
Smiles	NC1=C2C(=NN(CCCCNC(CCOCCOCCOCCOCCOCCOCCOCCOCCN3C=C(COCCO[C@H]4[C@H](OC)C[C@H](C[C@@H](C)[C@]5(OC(=O)[C@]6(N(C(=O)C(=O)[C@]7(O)O[C@@](CC[C@H]7C)(C[C@H](OC)\C(\C)=C\C=C\C=C/[C@@H](C)C[C@@H](C)C(=O)[C@H](OC)[C@H](O)/C(/C)=C\[C@@H](C)C(=O)C5)[H])CCCC6)[H])[H])CC4)N=N3)=O)C2=NC=N1)C=8C=C9C(=CC8)OC(N)=N9
Relative Density.	1.30 g/cm3 (Predicted)
Color	White
Appearance	Viscous

Storage & Solubility Information

Storage

keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 160 mg/mL (89.68 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 5 mg/mL (2.8 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	0.5605 mL	2.8025 mL	5.6049 mL	28.0247 mL
5 mM	0.1121 mL	0.5605 mL	1.1210 mL	5.6049 mL
10 mM	0.0560 mL	0.2802 mL	0.5605 mL	2.8025 mL
20 mM	0.0280 mL	0.1401 mL	0.2802 mL	1.4012 mL
50 mM	0.0112 mL	0.0560 mL	0.1121 mL	0.5605 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc