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OSMI-4

Name: OSMI-4
Brand: TargetMol
SKU: T12328
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12328Cas No. 2260791-14-6

OSMI-4 is a low nanomolar O-GlcNAc transferase (OGT) inhibitor (EC50 of 3 μM in cells) that can be used to study OGT inhibition in different human cell lines.

OSMI-4

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Purity: 99.68%

Catalog No. T12328Cas No. 2260791-14-6

OSMI-4 is a low nanomolar O-GlcNAc transferase (OGT) inhibitor (EC50 of 3 μM in cells) that can be used to study OGT inhibition in different human cell lines.

Pack Size	Price	Availability
1 mg	$215	In Stock
5 mg	$538	In Stock
10 mg	$778	In Stock
25 mg	$1,430	In Stock
50 mg	$2,450	In Stock
100 mg	$3,280	In Stock
500 mg	$4,360	6-8weeks
1 mL x 10 mM (in DMSO)	$593	In Stock

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This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. However, due to objective factors, there is a small probability that the synthesis may not be successful during the R&D process. We appreciate your understanding. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Purity:99.68%

ee:100%

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COA CSFC HNMR LCMS ORP

Product Introduction

Bioactivity

Description	OSMI-4 is a low nanomolar O-GlcNAc transferase (OGT) inhibitor (EC50 of 3 μM in cells) that can be used to study OGT inhibition in different human cell lines.
Targets&IC50	OGT:60 nM, OGT:3 μM (EC50)
In vitro	METHODS: HEK293T cells were treated with OSMI-4 (10 μM, 2 hours) and quantitative PCR was performed using primers for the OGT retained intron (intron 4) and spliced exon (exon 4). RESULTS The bar graph shows that OSMI-4 increases retained intron splicing. [1] METHODS: HEK293T cells were treated with OSMI-4 (20, 50 μM, 24 hours); HEK293T cells were treated with OSMI-4 (0.1, 1, 5, 5, 10, 20 μM, 2, 24, 48 hours); Western blot analysis of total O-GlcNAc was performed. RESULTS O-GlcNAc levels were reduced to a greater extent following OSMI-4 treatment; 24 hours of OSMI-4 treatment of HEK293T cells resulted in a dose-dependent decrease in O-GlcNAc levels, with effects seen with only 5 µM of compound treatment, and these effects could be observed in as little as 2 hours or 48 hours. [1]

Chemical Properties

Molecular Weight	604.09
Formula	C₂₇H₂₆ClN₃O₇S₂
Cas No.	2260791-14-6
Smiles	CCOC(=O)CN(Cc1cccs1)C(=O)[C@H](NS(=O)(=O)c1cc2ccc(=O)[nH]c2cc1Cl)c1ccccc1OC
Relative Density.	1.414 g/cm3 (Predicted)
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 252.5 mg/mL (417.98 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (8.28 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6554 mL	8.2769 mL	16.5538 mL	82.7691 mL
5 mM	0.3311 mL	1.6554 mL	3.3108 mL	16.5538 mL
10 mM	0.1655 mL	0.8277 mL	1.6554 mL	8.2769 mL
20 mM	0.0828 mL	0.4138 mL	0.8277 mL	4.1385 mL
50 mM	0.0331 mL	0.1655 mL	0.3311 mL	1.6554 mL
100 mM	0.0166 mL	0.0828 mL	0.1655 mL	0.8277 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc