N-Carbamoyl-L-aspartic acid

N-Carbamoyl-L-aspartic acid is an intermediate in pyrimidine metabolism and can be utilized for the measurement of dihydroorotase (DHOase) enzymatic activity. The metabolic role of N-Carbamoyl-L-aspartic acid supports the application of N-Carbamoyl-L-aspartic acid in studies of nucleotide biosynthesis, metabolic pathway characterization, enzymology, and biochemical assay development.

Method: Wild-type and pyrimidine-biosynthesis mutant strains of Saccharomyces cerevisiae were exposed to L-ureidosuccinic acid, and their growth and purine-biosynthetic responses were compared.
Rresult: L-Ureidosuccinic acid inhibited yeast growth by interfering with de novo purine biosynthesis at a step before the formation of 5-aminoimidazole ribonucleotide. Mutants blocked after dihydroorotate formation were more resistant than wild-type cells and mutants blocked at earlier steps of pyrimidine biosynthesis.[1]
Method: An in vitro one-pot multienzyme cascade containing carbamoyl phosphate synthase, aspartate transcarbamoylase, dihydroorotase, and dihydroorotate dehydrogenase was constructed to convert CO₂ and other feedstocks into N-carbamoyl-L-aspartate and orotate. Polyphosphate kinase was coupled to the system for ATP regeneration.
Rresult: Under the optimized conditions, the cascade produced 19.2 mM N-carbamoyl-L-aspartate within 3 h, enabling the subsequent production of 15.5 mM orotate.[2]
Method: An in vitro multienzyme cascade was constructed in which carbonic anhydrase hydrated CO₂ to bicarbonate, Pyrococcus furiosus carbamate kinase converted ammonia and bicarbonate into carbamoyl phosphate in an ATP-dependent reaction, and aspartate transcarbamoylase converted carbamoyl phosphate and L-aspartate into N-carbamoyl-L-aspartate.
Rresult: The one-pot multienzyme system remained highly active between 50 and 80°C and achieved an overall CO₂ conversion rate of 5.70%, confirming enzymatic production of N-carbamoyl-L-aspartate from ammonia and CO₂.[3]

Method: Human TNF-α transgenic arthritic mice were divided into control and Gancao Nourish-Yin Decoction groups. After 8 weeks of treatment, liver metabolites were analyzed using liquid chromatography–mass spectrometry.
Rresult: N-Carbamoyl-L-aspartic acid was identified as one of six significantly upregulated liver metabolites after treatment. Its abundance was positively correlated with creatinine and L-anserine, with correlation coefficients of 0.631 and 0.720, respectively.[4]
Method: Healthy rats received hydrogen-rich water or inhaled hydrogen gas for 6 months, after which plasma metabolites and fecal microbiota were analyzed using LC–MS-based pseudotargeted metabolomics and 16S rRNA sequencing.
Rresult: Hydrogen gas inhalation significantly increased plasma N-carbamoyl-L-aspartic acid compared with the control group. Its plasma abundance was moderately positively correlated with Paraprevotella and negatively correlated with Bifidobacterium.[5]
Method: Serum metabolomic profiles and gut microbial compositions were analyzed in an F6 pig population classified into high- and low-fatness groups.
Rresult: Serum N-carbamoyl-L-aspartic acid abundance was significantly lower in the high-fatness group than in the low-fatness group, with values of 874398.2 ± 257244.08 and 1107077.74 ± 284038.44, respectively; the p value was 0.017 and the VIP value was 1.409.[6]
Method: Milk samples from dairy cows receiving six corn-silage-based feeding systems were analyzed using untargeted high-resolution mass spectrometry to identify discriminant metabolites associated with dietary composition.
Rresult: N-Carbamoyl-L-aspartate was identified as a discriminant pyrimidine-biosynthesis intermediate with a VIP score of 1.25 ± 0.15. Its log2 fold changes in high-moisture-ear-corn-based diets versus feeding clusters 2, 3, and 5 were 0.86, 0.27, and −0.03, respectively.[7]

H2O: 25 mg/mL (141.94 mM), Sonication is recommended.