Monophosphoryl lipid A

Name: Monophosphoryl lipid A
Brand: TargetMol
SKU: T38636
Rating: 4.5 (1 reviews)

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Catalog No. T38636Cas No. 1246298-63-4

Alias Glucopyranosyl lipid A

Monophosphoryl lipid A (MPLA), also known as Glucopyranosyl lipid A, is an agonist of toll-like receptor 4. Derived from the cell wall of nonpathogenic Salmonella, MPLA is a valuable compound for research on immunization and vaccine development.

Monophosphoryl lipid A

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Catalog No. T38636Alias Glucopyranosyl lipid ACas No. 1246298-63-4

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$1,110	7-10 days	7-10 days

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Product Introduction

Bioactivity

Description	Monophosphoryl lipid A (MPLA), also known as Glucopyranosyl lipid A, is an agonist of toll-like receptor 4. Derived from the cell wall of nonpathogenic Salmonella, MPLA is a valuable compound for research on immunization and vaccine development.
In vitro	Monophosphoryl lipid A, at a concentration of 100 µg/ml for 2 hours, induces NF-κB activation and modulates TLR2 in dendritic cells, as evidenced by Western Blot Analysis. The same compound, ranging from 5 to 100 µg/ml over 24 hours, stimulates IL-12 production in human dendritic cells. Furthermore, increasing Monophosphoryl lipid A levels from 0.1 to 1 µg results in a higher percentage of matured bone marrow-derived dendritic cells (BMDCs). It also enhances cell surface markers on dendritic cells and boosts T cell responses, including the upregulation of calcium mobilization in activated CD4 T cells.
In vivo	The synthesized Monophosphoryl lipid A activates antigen-presenting cells (APCs) and amplifies the production of Th1- and Th2-specific immune responses in mice[2].
Synonyms	Glucopyranosyl lipid A

Chemical Properties

Molecular Weight	1763.501
Formula	C₉₆H₁₈₄N₃O₂₂P
Cas No.	1246298-63-4
Smiles	N.CCCCCCCCCCCCCC(=O)O[C@H](CCCCCCCCCCC)CC(=O)N[C@H]1[C@H](OC[C@H]2O[C@H](O)[C@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H]2O)O[C@H](CO)[C@@H](OP(O)(O)=O)[C@@H]1OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc