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ML334

Name: ML334
Brand: TargetMol
SKU: T12078
Price: 49 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12078Cas No. 1432500-66-7

Alias LH601A

ML334 (LH601A) is a transmembrane and potent NRF2 activator that inhibits Keap1-NRF2 protein interactions to ameliorate myocardial infarction-induced cardiac fibrosis by inhibiting cardiac fibroblast activation and proliferation.

ML334

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Purity: 98.06%

Catalog No. T12078Alias LH601ACas No. 1432500-66-7

Pack Size	Price	Availability	Quantity
1 mg	$49	In Stock

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Batch Information

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Purity:98.06%

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	ML334 (LH601A) is a transmembrane and potent NRF2 activator that inhibits Keap1-NRF2 protein interactions to ameliorate myocardial infarction-induced cardiac fibrosis by inhibiting cardiac fibroblast activation and proliferation.
Targets&IC50	Keap1:1 μM (Ki)
In vitro	Treatment of HEK293 cells with ML334 (LH601A; 50-100 μM; 6-16 hours) increases NQO1 and TRX1 mRNA levels by 2 to 3 times at both 6 and 16 hours. Additionally, at 6 hours, ML334 enhances HO-1 mRNA expression by 4 to 7 times, with a relatively smaller change in fold increase observed at 16 hours [2]. ML334 (LH601A; 50-100 μM; 16 hours; HEK293 cells) induces the expression of HO-1 and TRX1 proteins in HEK293 cells [2]. Furthermore, ML334 (LH601A) stimulates the expression and nuclear translocation of NRF2 in HEK293 cells [2].
Synonyms	LH601A

Chemical Properties

Molecular Weight	446.49
Formula	C₂₆H₂₆N₂O₅
Cas No.	1432500-66-7
Smiles	OC(=O)[C@H]1CCCC[C@H]1C(=O)N1CCc2ccccc2[C@H]1CN1C(=O)c2ccccc2C1=O
Relative Density.	1.354 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 30 mg/mL (67.19 mM), Sonication is recommended.

Ethanol: 30 mg/mL (67.19 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (11.2 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.2397 mL	11.1985 mL	22.3969 mL	111.9846 mL
5 mM	0.4479 mL	2.2397 mL	4.4794 mL	22.3969 mL
10 mM	0.2240 mL	1.1198 mL	2.2397 mL	11.1985 mL
20 mM	0.1120 mL	0.5599 mL	1.1198 mL	5.5992 mL
50 mM	0.0448 mL	0.2240 mL	0.4479 mL	2.2397 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc