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ML334

Name: ML334
Brand: TargetMol
SKU: T12078
Price: 49 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12078Cas No. 1432500-66-7

Alias LH601A

ML334 (LH601A) is a transmembrane and potent NRF2 activator that inhibits Keap1-NRF2 protein interactions to ameliorate myocardial infarction-induced cardiac fibrosis by inhibiting cardiac fibroblast activation and proliferation.

ML334

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Purity: 98.06%

Catalog No. T12078Alias LH601ACas No. 1432500-66-7

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$49	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98.06%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	ML334 (LH601A) is a transmembrane and potent NRF2 activator that inhibits Keap1-NRF2 protein interactions to ameliorate myocardial infarction-induced cardiac fibrosis by inhibiting cardiac fibroblast activation and proliferation.
Targets&IC50	Keap1:1 μM (Ki)
In vitro	Treatment of HEK293 cells with ML334 (LH601A; 50-100 μM; 6-16 hours) increases NQO1 and TRX1 mRNA levels by 2 to 3 times at both 6 and 16 hours. Additionally, at 6 hours, ML334 enhances HO-1 mRNA expression by 4 to 7 times, with a relatively smaller change in fold increase observed at 16 hours [2]. ML334 (LH601A; 50-100 μM; 16 hours; HEK293 cells) induces the expression of HO-1 and TRX1 proteins in HEK293 cells [2]. Furthermore, ML334 (LH601A) stimulates the expression and nuclear translocation of NRF2 in HEK293 cells [2].
Synonyms	LH601A

Chemical Properties

Molecular Weight	446.49
Formula	C₂₆H₂₆N₂O₅
Cas No.	1432500-66-7
Smiles	OC(=O)[C@H]1CCCC[C@H]1C(=O)N1CCc2ccccc2[C@H]1CN1C(=O)c2ccccc2C1=O
Relative Density.	1.354 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 30 mg/mL (67.19 mM), Sonication is recommended.

Ethanol: 30 mg/mL (67.19 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (11.2 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	2.2397 mL	11.1985 mL	22.3969 mL	111.9846 mL
5 mM	0.4479 mL	2.2397 mL	4.4794 mL	22.3969 mL
10 mM	0.2240 mL	1.1198 mL	2.2397 mL	11.1985 mL
20 mM	0.1120 mL	0.5599 mL	1.1198 mL	5.5992 mL
50 mM	0.0448 mL	0.2240 mL	0.4479 mL	2.2397 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc