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ML240

Name: ML240
Brand: TargetMol
SKU: T3535
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3535Cas No. 1346527-98-7

ML240 is a selective, ATP-competitive p97 inhibitor.

ML240

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Purity: 99.73%

Catalog No. T3535Cas No. 1346527-98-7

ML240 is a selective, ATP-competitive p97 inhibitor.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$32	In Stock	In Stock
2 mg	$45	In Stock	In Stock
5 mg	$74	In Stock	In Stock
10 mg	$113	In Stock	In Stock
25 mg	$198	In Stock	In Stock
50 mg	$328	In Stock	In Stock
100 mg	$529	-	In Stock
200 mg	$753	-	In Stock
1 mL x 10 mM (in DMSO)	$81	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.73%

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

ML240 AI Summary

ML240 exhibits multiple bioactivities. It inhibits human p97 ATPase with an IC50 of 815.0 nM, indicating its potential as a potent ATPase inhibitor. It displays cytotoxicity against human A549 cells with an IC50 of 3260.0 nM and against HepG2 cells with an IC50 of 9600.0 nM. In A549 cells, it leads to the accumulation of K48 poly-ubiquitinated proteins and CHOP poly-ubiquitinated proteins, with IC50 values of 6140.0 nM and 6610.0 nM respectively, implicating its role in protein degradation pathways. The compound also demonstrates antiviral activity, inhibiting SARS-CoV-2 in the Vero E6 cell line with an IC50 of approximately 476.43 nM. It shows good selectivity for viral inhibition over cytotoxicity, with an AUC ratio of 0.787. Additionally, the compound inhibits the p97 enzyme activity with an IC50 of 110.0 nM in a biomol green assay and shows reversible inhibition of His-tagged p97 enzyme and its C552A mutant, with 82.0% activity for wild-type p97 and complete inhibition for the C552A mutant at 100.0%. In testing against SARS-CoV-2 3CL-Pro protease at 20 µM, it shows an inhibition percentage of -7.72%. When tested on SARS-CoV-2 induced cytotoxicity in Vero-6 cells at 10 µM, it exhibits an inhibition of -0.16% after 48 hours. The compound has a short half-life of 0.1167 hours in mouse liver microsomes, indicating rapid metabolism, and a low aqueous solubility of less than 1.0 µg/mL, which may affect its formulation and delivery in aqueous solutions..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	ML240 is a selective, ATP-competitive p97 inhibitor.
Targets&IC50	p97:100 nM.

Chemical Properties

Molecular Weight	396.44
Formula	C₂₃H₂₀N₆O
Cas No.	1346527-98-7
Smiles	COc1cccc2c(NCc3ccccc3)nc(nc12)-n1c(N)nc2ccccc12
Relative Density.	1.35 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (126.12 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2.5 mg/mL (6.31 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5224 mL	12.6122 mL	25.2245 mL	126.1225 mL
5 mM	0.5045 mL	2.5224 mL	5.0449 mL	25.2245 mL
10 mM	0.2522 mL	1.2612 mL	2.5224 mL	12.6122 mL
20 mM	0.1261 mL	0.6306 mL	1.2612 mL	6.3061 mL
50 mM	0.0504 mL	0.2522 mL	0.5045 mL	2.5224 mL
100 mM	0.0252 mL	0.1261 mL	0.2522 mL	1.2612 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc