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ML239

Name: ML239
Brand: TargetMol
SKU: T4399
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4399Cas No. 1378872-36-6

Alias CID-49843203

ML239 (CID-49843203) is a potent and selective inhibitor of breast cancer stem cells, with an IC50 of 1.16 μM.

ML239

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Purity: 99.13%

Catalog No. T4399Alias CID-49843203Cas No. 1378872-36-6

ML239 (CID-49843203) is a potent and selective inhibitor of breast cancer stem cells, with an IC50 of 1.16 μM.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$30	In Stock	In Stock
25 mg	$60	In Stock	In Stock
50 mg	$117	In Stock	In Stock
100 mg	$200	In Stock	In Stock
200 mg	$293	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.13%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	ML239 (CID-49843203) is a potent and selective inhibitor of breast cancer stem cells, with an IC50 of 1.16 μM.
Targets&IC50	Breast cancer stem cells:1.16 μM.
In vitro	ML239 (Compound 7j) serves as a powerful and selective inhibitor targeting breast cancer stem cells, exhibiting an IC50 value of 1.16 μM and demonstrating approximately 24-fold greater selectivity compared to the control cell line. It operates primarily by potentially activating fatty acid desaturase 2 (FADS2), thereby inhibiting the growth of breast cancer stem-like cells. Additionally, ML239 shows cytotoxic effects on NCIH661 cells. Interestingly, the reduction of FADS2 activity, either through direct knockdown or by using the FADS2 inhibitor SC-26196, diminishes the cytotoxicity of ML239 in cancer cell lines (CCLs), suggesting a critical role of FADS2 in ML239's mechanism of action.
Kinase Assay	ML239 is dissolved in DMSO.Cancer cell lines (CCLs) are plated at a density of 500 cells/well in white opaque tissue-culture-treated Aurora 1536-well MaKO plates in the provider-recommended growth media using a highly automated platform. Compounds (ML239) are added by acoustic transfer using a Labcyte Echo 555. 24 hours after plating. The effects of small molecules (ML239) are measured over a 16-point concentration range (two-fold dilution) in duplicate. DMSO is used at a constant concentration of 0.33%, including vehicle-only control wells. As a surrogate for viability, cellular ATP levels are assessed 72 hours after compound transfer by addition of CellTiterGlo followed by luminescence measurement using a ViewLux Microplate Imager. Duplicates are averaged and luminescence values normalized to vehicle (DMSO) treatment and background (media-only) wells
Synonyms	CID-49843203

Chemical Properties

Molecular Weight	346.6
Formula	C₁₃H₁₀Cl₃N₃O₂
Cas No.	1378872-36-6
Smiles	Clc1cc(Cl)c(OCC(=O)N\N=C\c2ccc[nH]2)c(Cl)c1
Relative Density.	1.50 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (144.26 mM), Sonication is recommended.

Ethanol: 50 mM, Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2.5 mg/mL (7.21 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8852 mL	14.4259 mL	28.8517 mL	144.2585 mL
5 mM	0.5770 mL	2.8852 mL	5.7703 mL	28.8517 mL
10 mM	0.2885 mL	1.4426 mL	2.8852 mL	14.4259 mL
20 mM	0.1443 mL	0.7213 mL	1.4426 mL	7.2129 mL
50 mM	0.0577 mL	0.2885 mL	0.5770 mL	2.8852 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0289 mL	0.1443 mL	0.2885 mL	1.4426 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc