GP531 is a second-generation adenosine regulating agent. It is pharmacologically silent under basal conditions but increases localized endogenous adenosine during ischemia.

GP531 administration does not alter heart rate or mean aortic pressure but significantly improves left ventricular function by reducing end-diastolic pressure, volumes, and wall stress, while enhancing ejection fraction (EF), deceleration time of early mitral inflow velocity, and the slope of end-systolic pressure-volume relationship (PVR), without an increase in myocardial oxygen consumption (MVO2). At low doses, GP531 diminishes infarct size by 34% and the no-reflow zone extent by 31% compared to control, with high doses achieving reductions of 22% and 16%, respectively. Unlike adenosine, GP531 does not impact overall hemodynamics or blood flow, showcasing its efficacy in mitigating ischemic/reperfusion injury severity at lower doses without inducing adverse hemodynamic events such as bradycardia and hypotension.