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FIDAS-5

Name: FIDAS-5
Brand: TargetMol
SKU: T11285
Price: 54 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11285Cas No. 1391934-98-7

FIDAS-5 is an orally active methionine adenosyltransferase 2A (MAT2A) inhibitor with an IC50 of 2.1 μM. It can effectively compete with S-adenosylmethionine for MAT2A binding and has anticancer effects.

FIDAS-5

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Purity: 99.17%

Catalog No. T11285Cas No. 1391934-98-7

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$54	In Stock	In Stock
2 mg	$77	In Stock	In Stock
5 mg	$118	In Stock	In Stock
10 mg	$196	In Stock	In Stock
25 mg	$349	In Stock	In Stock
50 mg	$538	In Stock	In Stock
100 mg	$778	In Stock	In Stock
500 mg	$1,490	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.17%

Color:White to Yellow

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	FIDAS-5 is an orally active methionine adenosyltransferase 2A (MAT2A) inhibitor with an IC50 of 2.1 μM. It can effectively compete with S-adenosylmethionine for MAT2A binding and has anticancer effects.
Targets&IC50	Mat2A:2.1 μM
In vitro	METHODS: FIDAS-5 (3 μM; 36 hours) was used to treat LS174T cells, and the levels of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) were observed. RESULTS FIDAS-5 reduced S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) levels in LS174T cells. METHODS: FIDAS-5 (3 μM; 7 days) was used to treat LS174T cells, and the proliferation of LS174T cells was observed. RESULTS FIDAS-5 significantly inhibited the proliferation of LS174T cells. METHODS: FIDAS-5 (3 μM) treatment inhibited LS174T colorectal cancer cells, and the expression of c-Myc and cyclinD1 in LS174T colorectal cancer cells was observed by westernBlot. RESULTS FIDAS-5 inhibited the expression of c-Myc and cyclinD1 in LS174T colorectal cancer cells [1]. METHODS: OPM2 cells were transduced with siMAT2A or scrambled siRNA for 3 days. MAT2A-silenced and parental OPM2 cells were treated with different doses of FIDAS-5 (0.5, 1, 2 μM) to determine whether FIDAS-5 indeed impairs MM cell survival by inhibiting the enzymatic activity of MAT2A. RESULTS The antitumor effect of FIDAS-5 on MAT2A-silenced cells was impaired compared with cells transduced with scrambled siRNA. FIDAS-5 indeed inhibited MM cell survival in part by targeting MAT2A. [2]
In vivo	METHODS: Tumors were induced in athymic nude mice by subcutaneous injection of HT29 CRC cells. FIDAS-5 treatment (20 mg/kg) was administered orally via gavage. The oral efficacy of FIDAS-5 on HT29 tumor xenografts in nude mice was tested by measuring tumors twice weekly using digital calipers. RESULTS FIDAS-5 significantly inhibited xenograft tumor growth with minimal body weight differences. [1]

Chemical Properties

Molecular Weight	261.72
Formula	C₁₅H₁₃ClFN
Cas No.	1391934-98-7
Smiles	CNc1ccc(\C=C\c2c(F)cccc2Cl)cc1
Relative Density.	1.256 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 125 mg/mL (477.61 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (15.28 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8209 mL	19.1044 mL	38.2088 mL	191.0439 mL
5 mM	0.7642 mL	3.8209 mL	7.6418 mL	38.2088 mL
10 mM	0.3821 mL	1.9104 mL	3.8209 mL	19.1044 mL
20 mM	0.1910 mL	0.9552 mL	1.9104 mL	9.5522 mL
50 mM	0.0764 mL	0.3821 mL	0.7642 mL	3.8209 mL
100 mM	0.0382 mL	0.1910 mL	0.3821 mL	1.9104 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc