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Dihydrocytochalasin B

Name: Dihydrocytochalasin B
Brand: TargetMol
SKU: T11042
Rating: 4.5 (1 reviews)

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Catalog No. T11042Cas No. 39156-67-7

Alias Cytochalasin H2B

Dihydrocytochalasin B (H2CB) is a cell division inhibitor that prevents cytokinesis, alters cell morphology, disrupts actin structures in Swiss/3T3 mouse fibroblasts, and inhibits DNA synthesis induced by serum growth factors in quiescent cultures. Low doses of H2CB lead to cell rounding and the loss of actin microfilament bundles.

Dihydrocytochalasin B

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Catalog No. T11042Alias Cytochalasin H2BCas No. 39156-67-7

Pack Size	Price	USA Warehouse	Global Warehouse
500 μg	$282	35 days	35 days
1 mg	$533	35 days	35 days
5 mg	$2,090	35 days	35 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	Dihydrocytochalasin B (H2CB) is a cell division inhibitor that prevents cytokinesis, alters cell morphology, disrupts actin structures in Swiss/3T3 mouse fibroblasts, and inhibits DNA synthesis induced by serum growth factors in quiescent cultures. Low doses of H2CB lead to cell rounding and the loss of actin microfilament bundles.
Targets&IC50	P388/ADR cells:28 μM
In vitro	Dihydrocytochalasin B (2-10 X 10 (-7) M) disrupts the actin structure of Swiss/3T3 mouse fibroblasts and inhibits the ability of serum growth factor to stimulate DNA synthesis in static cultures. Low doses of H2CB cause cell rounding and loss of actin microfilaments, but they do not interfere with glucose or thymidine transport. [1] Dihydrocytochalasin B enhances reexpression of the phenotype of transformed growth factor-β induced differentiated chondrocytes without stimulating collagen synthesis. [2] Dihydrocytochalasin B (25 μg/mL) inhibited active calcium transport in intestinal absorption cells and led to increased Ca concentration in mucosal scrape. [3] The IC50 and IC80 of P388/ADR leukemia cell lines inhibited by Dihydrocytochalasin B were 28 μM and 48 μM, respectively. [4]
In vivo	Dihydrocytochalasin B (10 μM) is not toxic to zebrafish. Dihydrocytochalasin B is 20 times less toxic in mice than cytorelaxin D. [5]
Synonyms	Cytochalasin H2B

Chemical Properties

Molecular Weight	481.62
Formula	C₂₉H₃₉NO₅
Cas No.	39156-67-7
Smiles	O=C1[C@]23[C@]([C@H](CC4=CC=CC=C4)N1)([C@H](C)C(=C)[C@@H](O)[C@@]2(/C=C/C[C@H](C)CCC[C@@H](O)CCC(=O)O3)[H])[H]
Relative Density.	1.194g/cm3

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 20 mg/mL (41.53 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0763 mL	10.3816 mL	20.7633 mL	103.8163 mL
5 mM	0.4153 mL	2.0763 mL	4.1527 mL	20.7633 mL
10 mM	0.2076 mL	1.0382 mL	2.0763 mL	10.3816 mL
20 mM	0.1038 mL	0.5191 mL	1.0382 mL	5.1908 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc