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BRD4-IN-4

Name: BRD4-IN-4
Brand: TargetMol
SKU: T77339
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T77339Cas No. 304685-40-3

BRD4-IN-4 is a selective BRD4 inhibitor with an IC50 value of 6.83 μM for BRD4.BRD4-IN-4 selectively inhibits the proliferation of the MV4-11 cell line and arrests the cells in G1 phase.BRD4-IN-4 can be used to study MLL leukemias.

BRD4-IN-4

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Purity: 99.97%

Catalog No. T77339Cas No. 304685-40-3

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$31	In Stock	In Stock
25 mg	$50	In Stock	In Stock
50 mg	$74	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.97%

Appearance:Solid

Color:Green

Resource Download

COA HNMR LCMS

Product Introduction

BRD4-IN-4 AI Summary

BRD4-IN-4 is a potent inhibitor of the human BRD4-BD1 protein, targeting specific residues in the protein. It exhibits an IC50 value of 1910.0 nM in a specific substrate assay, indicating its potency. Moreover, it inhibits His-tagged BRD4 bromodomain-1 with an IC50 of 6830.0 nM and demonstrates strong inhibition (99.0%) at 20 uM, as well as 59.0% inhibition at 5 uM. The compound also shows binding affinity to the bromodomain, resulting in a Delta Tm of 3.4 degrees C in a thermal shift assay. Additionally, BRD4-IN-4 has antiproliferative effects on human MV4-11 cells with an IC50 of 11670.0 nM and induces cell cycle arrest at G1, G2, and S phases in a dose-dependent manner after 24 hours..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	BRD4-IN-4 is a selective BRD4 inhibitor with an IC50 value of 6.83 μM for BRD4.BRD4-IN-4 selectively inhibits the proliferation of the MV4-11 cell line and arrests the cells in G1 phase.BRD4-IN-4 can be used to study MLL leukemias.
Targets&IC50	BRD4:6.83 μM

Chemical Properties

Molecular Weight	330.4
Formula	C₁₇H₁₈N₂O₃S
Cas No.	304685-40-3
Smiles	CCN1C(=O)C2=CC=CC3=C(C=CC1=C23)S(=O)(=O)N1CCCC1

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (136.2 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.0266 mL	15.1332 mL	30.2663 mL	151.3317 mL
5 mM	0.6053 mL	3.0266 mL	6.0533 mL	30.2663 mL
10 mM	0.3027 mL	1.5133 mL	3.0266 mL	15.1332 mL
20 mM	0.1513 mL	0.7567 mL	1.5133 mL	7.5666 mL
50 mM	0.0605 mL	0.3027 mL	0.6053 mL	3.0266 mL
100 mM	0.0303 mL	0.1513 mL	0.3027 mL	1.5133 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc