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BN82002

Name: BN82002
Brand: TargetMol
SKU: T4671
Rating: 4.5 (1 reviews)

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Catalog No. T4671Cas No. 396073-89-5

Alias CDC25 Phosphatase Inhibitor I

BN82002 (CDC25 Phosphatase Inhibitor I) is a synthetic inhibitor of CDC25 phophatases

BN82002

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Purity: 98.24%

Catalog No. T4671Alias CDC25 Phosphatase Inhibitor ICas No. 396073-89-5

BN82002 (CDC25 Phosphatase Inhibitor I) is a synthetic inhibitor of CDC25 phophatases

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$38	6-8 weeks	6-8 weeks
5 mg	$89	6-8 weeks	6-8 weeks
10 mg	$122	6-8 weeks	6-8 weeks
25 mg	$222	6-8 weeks	6-8 weeks
50 mg	$372	6-8 weeks	6-8 weeks
100 mg	$556	6-8 weeks	6-8 weeks
1 mL x 10 mM (in DMSO)	$98	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.24%

Appearance:Viscous

Color:Black

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COA

Product Introduction

Bioactivity

Description	BN82002 (CDC25 Phosphatase Inhibitor I) is a synthetic inhibitor of CDC25 phophatases
Targets&IC50	CDC25B2:3.9 μM, CDC25A:2.4 μM, CDC25B3:6.3 μM, CDC25C:5.4 μM, CDC25C-cat:4.6 μM
In vitro	The in vitro impact of BN82002 on the proliferation of various human tumor cell lines has been assessed, with menadione serving as a control due to its documented proliferation inhibitory properties. All tested cell lines exhibit sensitivity to both BN82002 and menadione, demonstrating concentration-dependent inhibition within the low micromolar range. Among these, the pancreatic cancer cell line MIA PaCa-2 is the most affected by BN82002, showing an IC50 of 7.2 μM, while the colon cancer cell line HT-29 shows the least sensitivity with an IC50 of 32.6 μM. This range of inhibitory concentration for BN82002 closely matches that observed with menadione (5-15 μM). Further findings indicate that a 50 μM concentration of BN82002 completely halts cell proliferation. Although the influence on cell cycle distribution is relatively mild, there is a noted decrease in the S phase and an increase in cells with G1 and G2 DNA content, hinting at cell cycle arrest at various stages due to BN82002 treatment.
Synonyms	CDC25 Phosphatase Inhibitor I

Chemical Properties

Molecular Weight	359.42
Formula	C₁₉H₂₅N₃O₄
Cas No.	396073-89-5
Smiles	COc1cc(cc(CN(C)CCc2ccc(cc2)[N+]([O-])=O)c1O)N(C)C
Relative Density.	1.216 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 150 mg/mL (417.34 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (9.18 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7823 mL	13.9113 mL	27.8226 mL	139.1130 mL
5 mM	0.5565 mL	2.7823 mL	5.5645 mL	27.8226 mL
10 mM	0.2782 mL	1.3911 mL	2.7823 mL	13.9113 mL
20 mM	0.1391 mL	0.6956 mL	1.3911 mL	6.9557 mL
50 mM	0.0556 mL	0.2782 mL	0.5565 mL	2.7823 mL
100 mM	0.0278 mL	0.1391 mL	0.2782 mL	1.3911 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc