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BN82002

Name: BN82002
Brand: TargetMol
SKU: T4671
Rating: 4.5 (1 reviews)

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Catalog No. T4671Cas No. 396073-89-5

Alias CDC25 Phosphatase Inhibitor I

BN82002 (CDC25 Phosphatase Inhibitor I) is a synthetic inhibitor of CDC25 phophatases

BN82002

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Purity: 98.24%

Catalog No. T4671Alias CDC25 Phosphatase Inhibitor ICas No. 396073-89-5

BN82002 (CDC25 Phosphatase Inhibitor I) is a synthetic inhibitor of CDC25 phophatases

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$38	6-8 weeks	6-8 weeks
5 mg	$89	6-8 weeks	6-8 weeks
10 mg	$122	6-8 weeks	6-8 weeks
25 mg	$222	6-8 weeks	6-8 weeks
50 mg	$372	6-8 weeks	6-8 weeks
100 mg	$556	6-8 weeks	6-8 weeks
1 mL x 10 mM (in DMSO)	$98	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.24%

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COA

Product Introduction

Bioactivity

Description	BN82002 (CDC25 Phosphatase Inhibitor I) is a synthetic inhibitor of CDC25 phophatases
Targets&IC50	CDC25A:2.4 μM, CDC25B2:3.9 μM, CDC25B3:6.3 μM, CDC25C-cat:4.6 μM, CDC25C:5.4 μM
In vitro	The in vitro impact of BN82002 on the proliferation of various human tumor cell lines has been assessed, with menadione serving as a control due to its documented proliferation inhibitory properties. All tested cell lines exhibit sensitivity to both BN82002 and menadione, demonstrating concentration-dependent inhibition within the low micromolar range. Among these, the pancreatic cancer cell line MIA PaCa-2 is the most affected by BN82002, showing an IC50 of 7.2 μM, while the colon cancer cell line HT-29 shows the least sensitivity with an IC50 of 32.6 μM. This range of inhibitory concentration for BN82002 closely matches that observed with menadione (5-15 μM). Further findings indicate that a 50 μM concentration of BN82002 completely halts cell proliferation. Although the influence on cell cycle distribution is relatively mild, there is a noted decrease in the S phase and an increase in cells with G1 and G2 DNA content, hinting at cell cycle arrest at various stages due to BN82002 treatment.
Synonyms	CDC25 Phosphatase Inhibitor I

Chemical Properties

Molecular Weight	359.42
Formula	C₁₉H₂₅N₃O₄
Cas No.	396073-89-5
Smiles	COc1cc(cc(CN(C)CCc2ccc(cc2)[N+]([O-])=O)c1O)N(C)C
Relative Density.	1.216 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 150 mg/mL (417.34 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (9.18 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7823 mL	13.9113 mL	27.8226 mL	139.1130 mL
5 mM	0.5565 mL	2.7823 mL	5.5645 mL	27.8226 mL
10 mM	0.2782 mL	1.3911 mL	2.7823 mL	13.9113 mL
20 mM	0.1391 mL	0.6956 mL	1.3911 mL	6.9557 mL
50 mM	0.0556 mL	0.2782 mL	0.5565 mL	2.7823 mL
100 mM	0.0278 mL	0.1391 mL	0.2782 mL	1.3911 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc