This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
BN82002 hydrochloride
Catalog No. T38447 CAS
1049740-43-3
BN82002 hydrochloride is a potent, selective, and irreversible inhibitor of the CDC25 phosphatase family. It inhibits CDC25A, CDC25B2, CDC25B3, CDC25C, and 25C-cat with IC50 values of 2.4, 3.9, 6.3, 5.4, and 4.6 μM, respectively. Additionally, BN82002 hydrochloride exhibits approximately 20-fold greater selectivity over CD45 tyrosine phosphatase.
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BN82002 hydrochloride is a potent, selective, and irreversible inhibitor of the CDC25 phosphatase family. It inhibits CDC25A, CDC25B2, CDC25B3, CDC25C, and 25C-cat with IC50 values of 2.4, 3.9, 6.3, 5.4, and 4.6 μM, respectively. Additionally, BN82002 hydrochloride exhibits approximately 20-fold greater selectivity over CD45 tyrosine phosphatase.
The in vitro impact of BN82002 on the proliferation of various human tumor cell lines was assessed, employing Menadione, a known proliferation inhibitor, as a benchmark. BN82002 and Menadione were observed to inhibit proliferation across all tested cell lines in a concentration-dependent fashion within the low micromolar range. Particularly, the pancreatic cancer cell line MIA PaCa-2 demonstrated the highest sensitivity to BN82002 with an IC 50 of 7.2 μM, while the colon cancer cell line HT-29 exhibited the least sensitivity with an IC 50 of 32.6 μM. This sensitivity pattern closely mirrors that seen with Menadione (5-15 μM). Additionally, it was found that a 50 μM concentration of BN82002 effectively halted cell proliferation. However, this arrest saw only a slight shift in the cell cycle, characterized by a minor decrease in the S phase and a rise in cells at both G1 and G2 DNA content stages, indicating BN82002-induced arrest occurs at various cell cycle phases[1].
Molecular Weight
395.88
Formula
C19H26ClN3O4
CAS No.
1049740-43-3
Storage
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Method for preparing DMSO master liquid: mg
drug pre-dissolved in μL DMSO (Master liquid concentration
mg/mL),
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL PEG300, mix and clarify, next add μL
Tween 80,mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL Corn oil,mix and clarify.
Note:
Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.