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Ammonium chloride

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Catalog No. T64755Cas No. 12125-02-9

Ammonium chloride is a compound commonly used in molecular biology research. As a dipolar compound that regulates pH, it can induce intracellular alkalinization and metabolic acidosis, thereby affecting enzyme activity and biological processes. Additionally, ammonium chloride acts as an inhibitor of autophagy and lysosomal function, and can be used to establish kidney stone models.

Ammonium chloride

Ammonium chloride

🥰Excellent
Catalog No. T64755Cas No. 12125-02-9
Ammonium chloride is a compound commonly used in molecular biology research. As a dipolar compound that regulates pH, it can induce intracellular alkalinization and metabolic acidosis, thereby affecting enzyme activity and biological processes. Additionally, ammonium chloride acts as an inhibitor of autophagy and lysosomal function, and can be used to establish kidney stone models.
Pack SizePriceAvailabilityQuantity
100 mg$30In Stock
500 mg$55In Stock
1 g$80In Stock
5 g$189In Stock
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Product Introduction

Bioactivity
Description
Ammonium chloride is a compound commonly used in molecular biology research. As a dipolar compound that regulates pH, it can induce intracellular alkalinization and metabolic acidosis, thereby affecting enzyme activity and biological processes. Additionally, ammonium chloride acts as an inhibitor of autophagy and lysosomal function, and can be used to establish kidney stone models.
In vitro
Ammonium Chloride (NH4Cl) reduces the yield of reovirus during infection of mouse L cells.[2]
In vivo
Ammonium Chloride (0.28 M in drinking water; 8-9-week-old C57B/L6 mice) promotes the survival of myocardial cells in vivo by decreasing contractile dysfunction, cardiac hypertrophy, inflammation, apoptosis and autophagy. Ammonium Chloride effectively improved doxorubicin (DOX)-induced cardiomyocyte apoptosis and cardiac dysfunction in mice.[1]
Chemical Properties
Molecular Weight53.49
FormulaClH4N
Cas No.12125-02-9
SmilesCl[NH4]
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 18.33 mg/mL (342.74 mM), Sonication is recommended.
H2O: 80 mg/mL (1495.61 mM), Sonication is recommended.
Solution Preparation Table
DMSO/H2O
1mg5mg10mg50mg
1 mM18.6951 mL93.4754 mL186.9508 mL934.7542 mL
5 mM3.7390 mL18.6951 mL37.3902 mL186.9508 mL
10 mM1.8695 mL9.3475 mL18.6951 mL93.4754 mL
20 mM0.9348 mL4.6738 mL9.3475 mL46.7377 mL
50 mM0.3739 mL1.8695 mL3.7390 mL18.6951 mL
100 mM0.1870 mL0.9348 mL1.8695 mL9.3475 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

FungalAutophagy
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