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Ammonium chloride

Name: Ammonium chloride
Brand: TargetMol
SKU: T64755
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T64755 Copy Product Info

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Ammonium chloride is a compound commonly used in molecular biology research. As a dipolar compound that regulates pH, it can induce intracellular alkalinization and metabolic acidosis, thereby affecting enzyme activity and biological processes. Additionally, ammonium chloride acts as an inhibitor of autophagy and lysosomal function, and can be used to establish kidney stone models.

Ammonium chloride

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Catalog No. T64755

Cas No. 12125-02-9

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Pack Size	Price	USA Stock	Global Stock
200 mg	$35	In Stock	In Stock
500 mg	$55	In Stock	In Stock
1 g	$80	In Stock	In Stock
5 g	$139	-	In Stock
10 g	$172	-	In Stock
25 g	$288	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	Ammonium chloride is a compound commonly used in molecular biology research. As a dipolar compound that regulates pH, it can induce intracellular alkalinization and metabolic acidosis, thereby affecting enzyme activity and biological processes. Additionally, ammonium chloride acts as an inhibitor of autophagy and lysosomal function, and can be used to establish kidney stone models.
In vitro	Ammonium Chloride (NH4Cl) reduces the yield of reovirus during infection of mouse L cells.[2]
In vivo	Ammonium Chloride (0.28 M in drinking water; 8-9-week-old C57B/L6 mice) promotes the survival of myocardial cells in vivo by decreasing contractile dysfunction, cardiac hypertrophy, inflammation, apoptosis and autophagy. Ammonium Chloride effectively improved doxorubicin (DOX)-induced cardiomyocyte apoptosis and cardiac dysfunction in mice.[1]
Disease Modeling Protocol	Kidney stone model Modeling Mechanism： Ammonium chloride, when used in combination with 1% ethylene glycol, induces metabolic acidosis, promoting increased renal oxalate excretion while simultaneously lowering urine pH, reducing the dissolution of calcium oxalate crystals, and promoting their deposition in renal tubules. It also synergistically triggers oxidative stress (increased ROS, decreased SOD and GSH-Px activity) and renal epithelial cell apoptosis (upregulation of cleaved caspase-3), ultimately leading to the formation of calcium oxalate kidney stones. Related Products： Ammonium chloride (T64755) Modeling Method： Experimental Subject： Rats, Sprague-Dawley, Male, 230±20g, 6-8 weeks old Dosage and Administration Route： 1% ethylene glycol+2% ammonium chloride mixture, 2 mL/animal, Oral gavage Dosing Frequency and Duration Model： Once daily, for 28 consecutive days Validation： 1. Physiological indicators: Serum urea nitrogen (BUN) and creatinine (CR) levels were significantly elevated, indicating renal function impairment; 2. Pathological indicators: Von Kossa staining showed a large amount of black calcium oxalate crystal deposition in renal tubules; HE staining revealed inflammatory infiltration and structural damage in renal tissue; 3. Molecular and biochemical indicators: ROS and MDA content in renal tissue was elevated, while SOD and GSH-Px activities were decreased; Western blotting showed upregulated expression of NOX2, α-SMA, and cleaved caspase-3 proteins, and downregulated SOD2 expression; 4. Functional verification: RNA-seq detection showed that differentially expressed genes in the kidney were enriched in pathways related to glutathione metabolism and peroxidase, which are associated with stone formation. Precautions： After euthanizing the animal on day 28, the kidneys were quickly separated. References：Chu X,et,al. Phillyrin prevents calcium oxalate kidney stones through the PPARγ signaling pathway. Ren Fail. 2025 Dec;47(1):2486559.

Chemical Properties

Molecular Weight	53.49
Formula	ClH₄N
Cas No.	12125-02-9
Smiles	Cl[NH4]

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 52 mg/mL (972.14 mM), Sonication is recommended.

H2O: 80 mg/mL (1495.61 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (46.74 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	18.6951 mL	93.4754 mL	186.9508 mL	934.7542 mL
5 mM	3.7390 mL	18.6951 mL	37.3902 mL	186.9508 mL
10 mM	1.8695 mL	9.3475 mL	18.6951 mL	93.4754 mL
20 mM	0.9348 mL	4.6738 mL	9.3475 mL	46.7377 mL
50 mM	0.3739 mL	1.8695 mL	3.7390 mL	18.6951 mL
100 mM	0.1870 mL	0.9348 mL	1.8695 mL	9.3475 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc