Alizarin Complexone (Alizarin-3-methyliminodiacetic acid) is an inhibitor of Rous-associated virus 2 reverse transcriptase. Alizarin Complexone can be used as a calcium-tracer and a chelating agent for biological studies.

I. Solution preparation
1. Mother solution preparation: Dissolve Alizarin Complexone in DMSO to obtain the required mother solution, usually 1-10mM.
2. Working solution preparation: Dilute the mother solution with PBS/DMEM/H2O to the required working solution, usually with a concentration range of 1-10μM.
II. Calcium ion detection
1. Add Alizarin Complexone working solution to the cell culture medium and incubate for a certain period of time to allow it to enter the cells and bind to calcium ions in the cells.
2. Use a fluorescence microscope or fluorescence spectrometer to detect the fluorescence signal in the range of 550-600 nm.
3. According to the intensity of the fluorescence signal, calculate the change in calcium ion concentration.
II. Reverse transcriptase inhibition experiment:
1. Dissolve Alizarin Complexone in a suitable solvent, usually at a concentration of 1-10 μM.
2. Mix the dissolved Alizarin Complexone with the RCV-2 reverse transcriptase solution.
3. Evaluate the inhibitory effect of Alizarin Complexone on reverse transcriptase activity by measuring reverse transcriptase activity (e.g., by radiolabeling or changes in fluorescence signal).

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: Slightly soluble