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Cytoskeletal Signaling Microtubule Associated Vinflunine Tartrate

Vinflunine Tartrate

Catalog No. T6722   CAS 1201898-17-0

Vinflunine Tartrat is a new vinca alkaloid uniquely fluorinated with the properties of mitotic-arresting and tubulin-interacting activity.

Vinflunine Tartrate, CAS 1201898-17-0
Pack Size Availability Price/USD Quantity
2 mg Inquiry 72.00
5 mg Inquiry 129.00
10 mg Inquiry 233.00
25 mg Inquiry 419.00
50 mg Inquiry 791.00
1 mL * 10 mM (in DMSO) Inquiry 233.00
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Purity 98.0%
Biological Description
Chemical Properties
Storage & Solubility Information
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Description Vinflunine Tartrat is a new vinca alkaloid uniquely fluorinated with the properties of mitotic-arresting and tubulin-interacting activity.
Targets&IC50 microtubule : ic50 1.2μM
In vivo Intravenous treatment of mice with Vinflunine, immediately before and 2 day after Matrigel implantation, results in a dose-dependent inhibition of the bFGF-induced angiogenic response, compared with vehicle-treated animals. Inhibition of haemoglobin content is significant at 1.25, 2.5 and 5 mg/kg, with no effect at 0.63 mg/kg (P > 0.05). An ID50 value (dose which inhibits 50% of bFGF-induced neovascularisation) is calculated as 1 mg/kg. Low doses of Vinflunine reduce the number of experimental liver metastases by human LS174T colon cancer cell. A slight overall decrease in liver metastatic foci is already observed at the very low dose of 0.16 mg/kg Vinflunine, although maximal overall inhibition is reached at the maximal tolerated dose (MTD) of 20 mg/kg. [5]
Kinase Assay Determination of Microtubule Polymer Mass: Purified tubulin (17 μM) is polymerized into microtubules in the abence or presence of a range of vnflunine concentrations (35 minutes; 37 °C) in 75 mM PIPES, 1.8 mM MgCl2, 1.0 mM EGTA, and 1.5 mM GTP (pH 6.8) using sea urchin (Strongylocentrotus purpuratus) axonemes as seeds for assembly initiation. After incubation, polymerized microtubules are separated from unpolymerized tubulin by centrifugation (150,000 × g; 1 hour; 35 °C). The supernatant is aspirated, the sedimented microtubules are depolymerized in assembly buffer by incubation on ice (2 hours), and the protein content is determined.
Cell Research
Effects of Vinflunine on L1210 cell proliferation are determined using a standard growth inhibition assay. Exponentially growing L1210 cells (1.5 × 105 cells/well) in a 24-well plate are exposed to a range of concentrations of test compounds for 48 hours, prior to determining cell numbers using an electronic particle counter based on linear interpolation between data points.(Only for Reference)
Cell lines: Leukemic L1210 cell
Animal Research
Animal Model: LS174T tumor cells are injected into the spleen of BALB/C nude mice.
Molecular Weight N/A
Formula C45H54F2N4O8·xC4H6O6
CAS No. 1201898-17-0

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 45 mg/mL (46.5 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

Saline: 30 mg/mL

Citations

References and Literature
1. Ngan VK, et al, Cancer Res, 2000, 60(18), 5045-5051. 2. Lobert S, et al, Biochemistry, 2000, 39(39), 12053-12062. 3. Okauneva T, et al, Mol Cancer Ther, 2003, 2(5), 427-436. 4. Pourroy B, et al, Mol Pharmacol, 2004, 66(3), 580-591. 5. Kruczynski A, et al, Eur J Cancer, 2006, 42(16), 2821-2832.

Related Products

Related compounds with same targets
Tubulin inhibitor 6 Deoxypodophyllotoxin Verubulin hydrochloride BO-264 Vinflunine Tartrate SSE15206 VERU-111 Verubulin

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