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VCMMAE

Catalog No. T4232 CAS 646502-53-6

Synonyms: mc-vc-PAB-MMAE

VCMMAE (mc-vc-PAB-MMAE) is a drug-linker conjugate for ADC with potent antitumor activity.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

VCMMAE, CAS 646502-53-6

Pack Size	Availability	Price/USD
5 mg	In stock	$ 48.00
10 mg	In stock	$ 91.00
25 mg	In stock	$ 166.00
50 mg	In stock	$ 265.00
100 mg	In stock	$ 397.00

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Purity: 100%

Purity: 99.58%

Purity: 99.27%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	VCMMAE (mc-vc-PAB-MMAE) is a drug-linker conjugate for ADC with potent antitumor activity.
In vitro	MMAE sensitized colorectal and pancreatic cancer cells to IR in a schedule and dose dependent manner correlating with mitotic arrest. Monomethyl auristatin E (MMAE) is efficiently released from SGN-35 within CD30+ cancer cells and, owing to its membrane permeability, it has the ability to exert cytotoxic activity on bystander cells. Radiosensitization is evidenced by decreased clonogenic survival and increased DNA double strand breaks in irradiated cells.
In vivo	Monomethyl auristatin E (MMAE) in combination with IR results in increased DNA damage signaling and activation of CHK1 that delay the tumor growth. Tumor-targeted ACPP-cRGD-MMAE with IR produces a more robust and significantly prolonged tumor regression in xenograft models.
Cell Research	Monomethyl auristatin E is reconstituted in DMSO at a concentration of 5 nM. Monomethyl auristatin E (MMAE, 5 nM) and ionizing radiation (IR) treated cells are harvested and lysed in RIPA buffer with protease and phosphatase inhibitors. 30 μg of lysate undergo electrophoresis using 4-12% Bis-Tris gels, transferred to PVDF membranes and incubated with indicated primary antibodies.
Animal Research	6-8 week old female athymic nu/nu mice are injected subcutaneously into thighs with 5×106 HCT-116 or PANC-1 cells in a 1:1 Matrigel and PBS solution. Mice are treated with IR or intravenous (IV) injection of ACPP-cRGD-MMAE (6 nmoles/day, 18 nmoles total, i.v.), tumor tissue is harvested, formalin fixed and paraffin embedded followed by staining with indicated antibodies. The primary antibody is used at a 1:250 dilution and is visualized using DAB as a chromagen with the UltraMap system.

Synonyms	mc-vc-PAB-MMAE
Molecular Weight	1316.63
Formula	C68H105N11O15
CAS No.	646502-53-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 54 mg/mL

References and Literature

1. Okeley, et al. Intracellular Activation of SGN-35, a Potent Anti-CD30 Antibody-Drug Conjugate. Clinicalal Cancer Research (2010), 16(3), 888-897. 2. Lisa Buckel, et al. Tumor radiosensitization by monomethyl auristatin E: mechanism of action and targeted delivery. Cancer Res. 2015 Apr 1;75(7):1376-87. 3. Jianmin Fang, et al. Anti-her2 antibody and conjugate thereof. US 20160304621 A1.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library NO PAINS Compound Library Cytoskeletal Signaling Pathway Compound Library Bioactive Compounds Library Max Bioactive Compound Library Anti-Cancer Compound Library

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Related compounds with same targets

Mebendazole SS28 Terflavoxate MPT0B014 Thiocolchicine AZ82 Tubulin polymerization-IN-55 CHM-1

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Keywords

VCMMAE 646502-53-6 Cytoskeletal Signaling Microtubule Associated mc-vc-PAB-MMAE inhibit Drug-Linker Conjugates for ADC Inhibitor Microtubule/Tubulin inhibitor

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