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PF04929113

Catalog No. T6341 CAS 908115-27-5

Synonyms: PF-04929113, SNX-5422, PF 04929113

PF04929113 (SNX-5422) is a synthetic, novel, small molecule Hsp90 Inhibitor. As an oral formulation that demonstrates strong efficacy and tolerability, PF04929113 is positioned as a breakthrough therapy with broad applicability across a wide range of cancers.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

PF04929113, CAS 908115-27-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 33.00
2 mg	In stock	$ 45.00
5 mg	In stock	$ 76.00
10 mg	In stock	$ 122.00
25 mg	In stock	$ 197.00
50 mg	In stock	$ 372.00
100 mg	In stock	$ 555.00
1 mL * 10 mM (in DMSO)	In stock	$ 88.00

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Purity: 99.06%

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Description	PF04929113 (SNX-5422) is a synthetic, novel, small molecule Hsp90 Inhibitor. As an oral formulation that demonstrates strong efficacy and tolerability, PF04929113 is positioned as a breakthrough therapy with broad applicability across a wide range of cancers.
Targets&IC50	HSP90:41 nM, HER2:37 nM
Kinase Assay	The Hsp90 loaded media is challenged with PF-04929113 at a given concentration, ranging from 0.8 to 500 μM, and the amount of Hsp90 liberated at each concentration is determined by Bradford protein assay. The resulting IC50 values are corrected for the ATP ligand concentration and presented as apparent Kd values.
Cell Research	Cell lines: MCF-7,SW620,K562,SK-MEL-5 and A375 cancer cell lines. Concentrations: 0-300 nM. Method: Proliferation rates are measured by seeding cells into 96-well plates,followed by compound addition 24 h later.After addition of PF-04929113,cells are allowed to grow for either an additional 72 or 144 h depending on the rate of growth.At harvest,media is removed and DNA content for individual wells is determined using CyQuant DNA dye.Levels of Hsp90 client proteins and phosphor-regulated proteins in A375 are measured by high content analysis (HCA) using an ArrayScan 4.5 instrument after 24 hours of treatment with PF-04929113,followed by methanol fixation.After fixation in 4% PBS-buffered formalin and permeablization with 0.1% TX-100,cells are probed with anti-Her2,antiphospho-S6 (pS6),antipERK,and anti-Hsp70 primary antibodies,followed by TRITC or FITC conjugated secondary antibodies.Nuclei are also stained with Hoechst DNA binding dye.For each well,250-500 individual nuclei are identified along with the average staining intensity for the client and phospho-proteins for each cell.Average client staining intensities are then calculated for each well.
Animal Research	Animal Models: 5 ×106 MM.1S cells are inoculated subcutaneously in the Fox Chase SCID mice (6-7 weeks old). Formulation: PF-04929113 is dissolved in 1% carboxy methylcellulose/0.5% Tween 80 at 10 mg/mL and stored at 4 °C. Dosages: 20 or 40 mg/kg. Administration: orally 3 times per week,3 weeks in total.

Synonyms	PF-04929113, SNX-5422, PF 04929113
Molecular Weight	521.53
Formula	C25H30F3N5O4
CAS No.	908115-27-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 3 mg/mL(5.8 mM)

DMSO: 96 mg/mL (184.1 mM)

References and Literature

1. Huang KH, et al, J Med Chem, 2009, 52(14), 4288-4305 2. Yutaka Okawa, et al, Blood, 2009, 113(4), 846-855. 3. Lamoureux F, et al, Clin Cancer Res, 2011, 17(8), 2301-2313.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Anti-Cancer Clinical Compound Library Tyrosine Kinase Inhibitor Library Inhibitor Library Anti-Cancer Drug Library Drug Repurposing Compound Library Anti-Aging Compound Library Anti-Pancreatic Cancer Compound Library ReFRAME Related Library Anti-Prostate Cancer Compound Library

Related Products

Related compounds with same targets

Paeoniflorin HSP90-IN-22 VER-155008 HSP70/SIRT2-IN-2 HSP70-IN-1 KNK437 CCT018159 Arimoclomol

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Keywords

PF04929113 908115-27-5 Angiogenesis Cytoskeletal Signaling JAK/STAT signaling Metabolism Tyrosine Kinase/Adaptors HSP HER PF-04929113 SNX5422 SNX-5422 SNX 5422 PF 04929113 inhibitor inhibit

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