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Monomethyl auristatin E

Catalog No. T6897   CAS 474645-27-7
Synonyms: Vedotin, MMAE

Monomethyl auristatin E (MMAE) (MMAE), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin and also has inhibition of antibody-drug conjugates (ADCs) activity.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Monomethyl auristatin E Chemical Structure
Monomethyl auristatin E, CAS 474645-27-7
Pack Size Availability Price/USD Quantity
10 mg In stock $ 41.00
25 mg In stock $ 64.00
50 mg In stock $ 91.00
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Purity: 99.66%
Purity: 99.47%
Purity: 98.47%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Monomethyl auristatin E (MMAE) (MMAE), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin and also has inhibition of antibody-drug conjugates (ADCs) activity.
In vitro When coupled to cAC10, MMAE shows selective cytotoxicity in CD30+ cells, and induces G2/M-phase growth arrest and cell death through the induction of apoptosis. [1] When coupled to anti-CD79b antibody, anti–CD79b-vcMMAE has very potent and broad activity across a large panel of NHL cell lines in vitro. [2] When coupled to anti-HER2 antibody, hertuzumab-vc-MMAE can also be effectively internalized and potently kill HER2 over-expressing tumor cells. [3]
In vivo In the Karpas 299 ALCL model, cAC10-vcMMAE (1 mg/kg, i.v.) induces complete, durable tumor regression, while free MMAE (0.36 mg/kg) doesn't produce detectable antitumor activity. [1] In mouse xenograft models of NHL, anti–CD79b-vcMMAE (7 mg/kg, p.o.) strikingly results in sustained complete tumor remission. [2]
Cell Research Cytotoxicity is measured using Alamar Blue dye reduction assay according to the manufacturer's directions. Briefly, a 40% solution (wt/vol) of Alamar Blue is freshly prepared in complete media just before cultures are added. Ninety-two hours after drug exposure, Alamar Blue solution is added to cells to constitute 10% culture volume. Cells are incubated for 4 hours, and dye reduction is measured on a Fusion HT fluorescent plate reader (Packard Instruments, Meriden, CT). (Only for Reference)
Synonyms Vedotin, MMAE
Molecular Weight 717.98
Formula C39H67N5O7
CAS No. 474645-27-7

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 71.8 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

TargetMolReferences and Literature

1. Francisco JA, et al. Blood. 2003, 102(4), 1458-1465. 2. Dornan D, et al. Blood. 2009, 114(13), 2721-2729. 3. Yao X, et al. Breast Cancer Res Treat. 2015, 153(1), 123-133.

TargetMolCitations

1. Ai Y, Wang W, Liu F, et al.Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P.Cell Research.2023: 1-19.

Related compound libraries

This product is contained In the following compound libraries:
Microtubule-Targeted Compound Library Anti-Cancer Active Compound Library Anti-Cancer Compound Library Bioactive Compound Library Cytoskeletal Signaling Pathway Compound Library Drug-induced Liver Injury (DILI) Compound Library Inhibitor Library Target-Focused Phenotypic Screening Library Apoptosis Compound Library Preclinical Compound Library

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Keywords

Monomethyl auristatin E 474645-27-7 Apoptosis Cytoskeletal Signaling Microtubule Associated SGD1010 Microtubule/Tubulin SGD 1010 Vedotin inhibit SGD-1010 MMAE Inhibitor ADC Payload ADC Cytotoxin inhibitor

 

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