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MAFP

Catalog No. T15948 CAS 188404-10-6

Synonyms: Methyl Arachidonyl Fluorophosphonate

MAFP is an effective irreversible inhibitor of anandamide amidase. MAFP is an active-site directed and irreversible inhibitor of cPLA2 and iPLA2.

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MAFP, CAS 188404-10-6

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

MAFP is an effective irreversible inhibitor of anandamide amidase. MAFP is an active-site directed and irreversible inhibitor of cPLA2 and iPLA2.

In vitro

In a concentration-dependent manner after a 5 min preincubation at 40°C in P388D1 cells, MAFP inhibits iPLA2 (IC50: 5 μM). MAFP is also an inhibitor of anandamide amidase and as a ligand for the CB1 cannabinoid receptor. MAFP shows selectivity towards anandamide amidase for which it is approximately 3000 and 30000-fold more potent than it is towards chymotrypsin and trypsin, respectively. cPLA is a phospholipid hydrolase using the hydroxyl of serine-228 residue as its catalytic nucleophile[1]. MAFP displaces [3H]CP-55940 binding to the CB1 cannabinoid receptor with an IC50 of 20 nM vs 40 nM for anandamide[2].

Synonyms	Methyl Arachidonyl Fluorophosphonate
Molecular Weight	370.48
Formula	C21H36FO2P
CAS No.	188404-10-6

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Methyl Acetate: 9.0 mg/mL (24.3 mM)

DMSO: 90.0 mg/mL (242.9 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Lio YC, et al. Irreversible inhibition of Ca(2+)-independent phospholipase A2 by methyl arachidonyl fluorophosphonate. Biochim Biophys Acta. 1996 Jul 12;1302(1):55-60. 2. Deutsch DG, et al. Methyl arachidonyl fluorophosphonate: a potent irreversible inhibitor of anandamide amidase. Biochem Pharmacol. 1997 Feb 7;53(3):255-60.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

MAFP 188404-10-6 其他 Others Methyl Arachidonyl Fluorophosphonate Inhibitor inhibitor inhibit

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