store at low temperature
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dihydromunduletone (DHM) is an adhesion G protein-coupled receptor (aGPCR) antagonist that acts as a chemical probe for the inhibition of GPR56 and GPR114/ADGRG5, which have similar tethered agonists.
Pack Size | Availability | Price/USD | Quantity |
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1 mg | In stock | $ 179.00 |
Description | Dihydromunduletone (DHM) is an adhesion G protein-coupled receptor (aGPCR) antagonist that acts as a chemical probe for the inhibition of GPR56 and GPR114/ADGRG5, which have similar tethered agonists. |
Targets&IC50 | GPR56:20.9 μM |
In vitro |
Assays are initiated by the addition of [35S]GTPγS, and the rates of aGPCR-stimulated G protein activation (Gα binding to [35S]GTPγS) are measured with or without the influence of added compounds. Dihydromunduletone (DHM) inhibits the kinetics of GPR56 7TM-stimulated G13 GTPγS binding to varying degrees. Among the inhibitory compounds tested, Dihydromunduletone is the most effective, reducing the rate at which GPR56 7TM activates G13 by over 75% (from 0.18 to 0.04 minute−1)[1].At a concentration of Dihydromunduletone (DHM) that maximally inhibits GPR56 (50 μM), the rate of GPR114 7TM-stimulated Gs activity is also dramatically inhibited. However, when Dihydromunduletone (50 μM) is applied to GPR110 7TM, it fails to inhibit GPR110 stimulation of Gq GTPγS binding[1]. Cells transfected with GPR56 A386M 7TM are incubated with increasing concentrations of Dihydromunduletone. After adding the P7 peptide agonist, SRE-luciferase activity is measured. Dihydromunduletone inhibits P7 peptide-induced luciferase activity in a concentration-dependent manner. Additionally, cells treated with a fixed concentration of 3 µM Dihydromunduletone and then stimulated with an increasing concentration of P7 peptide agonist show that Dihydromunduletone treatment blunts P7 peptide activation at each concentration. In conclusion, Dihydromunduletone antagonizes synthetic-peptide agonist and tethered-peptide agonist-mediated aGPCR activation in isolated membranes and HEK293T cell-based assays, but it does not inhibit basal receptor signaling[1]. |
Synonyms | DHM |
Molecular Weight | 424.49 |
Formula | C25H28O6 |
CAS No. | 674786-20-0 |
store at low temperature
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 20 mg/mL (47.12 mM), Sonication is recommended.
You can also refer to dose conversion for different animals. More
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Dihydromunduletone 674786-20-0 Endocrinology/Hormones GPCR/G Protein GPR DHM inhibitor inhibit