Shopping Cart

Remove All

Your shopping cart is currently empty

Proceed to checkout Continue shopping

2-Methylcitric acid

Name: 2-Methylcitric acid
Brand: TargetMol
SKU: T10076
Rating: 4.5 (1 reviews)

🥰Excellent

Catalog No. T10076Cas No. 6061-96-7

Alias Methylcitric acid

2-Methylcitric acid (Methylcitric acid) is a metabolic marker of methylmalonic acidemia that induces mitochondrial permeability transition (PT) and reduces ATP production in brain mitochondria.

2-Methylcitric acid

🥰Excellent

Catalog No. T10076Alias Methylcitric acidCas No. 6061-96-7

2-Methylcitric acid (Methylcitric acid) is a metabolic marker of methylmalonic acidemia that induces mitochondrial permeability transition (PT) and reduces ATP production in brain mitochondria.

Product information is being updated, if you want to purchase, please click the bulk custom button.

Add to Quotation

Questions

Batch Information

Select Batch

Purity:≥97.0%

Appearance:Viscous

Color:Black

Resource Download

COA

Product Introduction

Bioactivity

Description	2-Methylcitric acid (Methylcitric acid) is a metabolic marker of methylmalonic acidemia that induces mitochondrial permeability transition (PT) and reduces ATP production in brain mitochondria.
In vitro	2-Methylcitric acid has a significant effect on glutamate metabolism and interferes with mitochondrial function through a variety of mechanisms. It inhibits glutamate dehydrogenase (GDH) activity at concentrations as low as 0.5 mM, which in turn affects glutamate oxidation. In addition, 2-Methylcitric acid acts on mitochondria at concentrations of 1-3 mM, causing a significant decrease in membrane potential and mitochondrial swelling, a process that is caused by its ability to inhibit calcium loading supported by different substrates.2-Methylcitric acid has been shown to interfere with mitochondrial function by inhibiting glutamate dehydrogenase (GDH) activity at concentrations as low as 0.5 mM. The compound disrupts mitochondrial energy homeostasis by inhibiting glutamate dehydrogenase activity and acting as an inducer of permeability transition. Notably, although 2-Methylcitric acid disrupts mitochondrial energy homeostasis, it does not significantly affect glutamate transport function in liver mitochondria. [1]
Synonyms	Methylcitric acid

Chemical Properties

Molecular Weight	206.15
Formula	C₇H₁₀O₇
Cas No.	6061-96-7
Smiles	O=C(O)CC(O)(C(=O)O)C(C(=O)O)C
Relative Density.	1.178g/cm3

Storage & Solubility Information

Storage

store at low temperature | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (388.07 mM), Sonication is recommended.

H2O: 80 mg/mL (388.07 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (16.01 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	4.8508 mL	24.2542 mL	48.5084 mL	242.5418 mL
5 mM	0.9702 mL	4.8508 mL	9.7017 mL	48.5084 mL
10 mM	0.4851 mL	2.4254 mL	4.8508 mL	24.2542 mL
20 mM	0.2425 mL	1.2127 mL	2.4254 mL	12.1271 mL
50 mM	0.0970 mL	0.4851 mL	0.9702 mL	4.8508 mL
100 mM	0.0485 mL	0.2425 mL	0.4851 mL	2.4254 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc