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(-)-Blebbistatin

(-)-Blebbistatin
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Purity:99.59%
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(-)-Blebbistatin

Catalog No. T6038Cas No. 856925-71-8
(-)-Blebbistatin ((S)-(-)-Blebbistatin) is an S enantiomer of blebbistatin. It is a potent and selective myosin II inhibitor with IC50 ranging from 0.5 to 5 μM.
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Pack SizePriceAvailabilityQuantity
2 mg$35In Stock
5 mg$57In Stock
10 mg$97In Stock
25 mg$189In Stock
50 mg$297In Stock
100 mg$478In Stock
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Product Introduction

Bioactivity
Description
(-)-Blebbistatin ((S)-(-)-Blebbistatin) is an S enantiomer of blebbistatin. It is a potent and selective myosin II inhibitor with IC50 ranging from 0.5 to 5 μM.
In vitro
METHODS: Mouse hepatic stellate cell HSCs were treated with (-)-Blebbistatin (12.5-50 µM) for 2 h and cell morphology was observed using microscopy.
RESULTS: When treated with (-)-Blebbistatin for 2 h, the cells became smaller, acquired more dendritic morphology, and had fewer stress fibers and lamellipods. [1]
METHODS: Porcine TM cells were treated with (-)-Blebbistatin (25-100 µM) for 2 h. The expression levels of target proteins were detected using Western Blot.
RESULTS: There was no difference in MLC phosphorylation status between control and drug-treated samples. [2]
In vivo
Blebbistatin completely relaxes both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction in a dose-dependent manner. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4].
Kinase Assay
Inhibition of Cox Activity: Inhibition of Cox-1 activity is measured by monitoring oxygen consumption during the conversion of arachidonic acid to PGs using a Clark-type O2-electrode. The reaction mixture contains ~0.2 units Cox-1 in 100 μL of microsome fraction derived from ram seminal vesicles as a crude source of Cox-1 (specific activity 0.2–1 units/mg protein) or 0.23 units of recombinant human Cox-2 (specific activity 43 units/mg protein). For calculation, the rate of O2 consumption is compared with a DMSO control (100% activity). Piroxicam, a nonsteroidal anti-inflammatory drug, is used as positive inhibitory substance for Cox-1 activity with an IC50 of 0.35 ± 0.05 μM (n = 2). Alternatively, nimesulide, a Cox-2 specific inhibitor, inhibits Cox-2 activity by 52 ± 5.7% (n = 2) at a concentration of 50 μM.
Cell Research
Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3].
Alias(S)-(-)-Blebbistatin
Chemical Properties
Molecular Weight292.33
FormulaC18H16N2O2
Cas No.856925-71-8
Storage & Solubility Information
Storage Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 55 mg/mL (188.14 mM)
5% DMSO+95% Saline: 1.46 mg/mL (4.99 mM, precipitation)
Solution Preparation Table
DMSO/5% DMSO+95% Saline
1mg5mg10mg50mg
1 mM3.4208 mL17.1040 mL34.2079 mL171.0396 mL
DMSO
1mg5mg10mg50mg
5 mM0.6842 mL3.4208 mL6.8416 mL34.2079 mL
10 mM0.3421 mL1.7104 mL3.4208 mL17.1040 mL
20 mM0.1710 mL0.8552 mL1.7104 mL8.5520 mL
50 mM0.0684 mL0.3421 mL0.6842 mL3.4208 mL
100 mM0.0342 mL0.1710 mL0.3421 mL1.7104 mL

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