Loaded Human NRG1 Beta (245AA) on APS Biosensor, can bind Human HER3-mFc with an affinity constant of 3.6 nM as determined in BLI assay.
Loaded Human NRG1Beta (246AA) on APS Biosensor, can bind Human HER3-mFc with an affinity constant of 4.3 nM as determined in BLI assay.

HER3/ERBB3 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 95 kDa and the accession number is P21860.

Human

HEK293 Cells

p85-sErbB3,p45-sErbB3,p180-ErbB3,MDA-BF-1,LCCS2,HER3,erbB3-S,ErbB-3,erb-b2 receptor tyrosine kinase 3,EEBB3,c-erbB-3,c-erbB3

Ser20-Thr643

> 95% as determined by SDS-PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy