CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated

CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

Pack Size	Availability	Price/USD	Quantity
20 μg	5 days	$ 355.00
100 μg	5 days	$ 963.00

Pack Size

Availability

Price/USD

Quantity

20 μg

5 days

$ 355.00

100 μg

5 days

$ 963.00

Description

Species	Human
Expression System	Human Cells
Tag	N-6His-Avi
Accession Number	P29965
Synonyms	CD40 Ligand, TNF-Related Activation Protein, CD40LG, TNFSF5, CD40L, CD40-L, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154, T-Cell Antigen Gp39, TRAP
Amino Acid	Glu108-Leu261
Construction	Biotinylated Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Glu108-Leu261 is expressed with a 6His, Avi tag at the N-terminus.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	23-28 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

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CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated

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