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CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated

(Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 5, TRAP, TNFSF5, TNF-Related Activation Protein, T-Cell Antigen Gp39, CD40LG, CD40-L, CD40L, CD40 Ligand, CD154) Copy Product Info

Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 5, TRAP, TNFSF5, TNF-Related Activation Protein, T-Cell Antigen Gp39, CD40LG, CD40-L, CD40L, CD40 Ligand, CD154

Catalog No. TMPJ-00152 Copy Product Info
CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-6xHis-Avi tag. The predicted molecular weight is 23-28 KDa and the accession number is P29965.

Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-6xHis-Avi tag. The predicted molecular weight is 23-28 KDa and the accession number is P29965.
Species
Human
Expression System
HEK293 Cells
TagN-6xHis-Avi
Accession NumberP29965
Amino AcidGlu108-Leu261
ConstructionGlu108-Leu261
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 1 mM EDTA, pH 7.4.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsTumor Necrosis Factor Ligand Superfamily Member 5, TRAP, TNFSF5, TNF-Related Activation Protein, T-Cell Antigen Gp39, CD40LG, CD40-L, CD40L, CD40 Ligand, CD154
Research Background
CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Chemical Properties
Molecular Weight23-28 KDa (reducing condition)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | CD40 Ligand Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight