Home Tools

Zoledronic acid disodium tetrahydrate

Catalog No. T61764 CAS 165800-07-7

Zoledronic Acid (Zoledronate) disodium tetrahydrate is a potent third-generation bisphosphonate (BP), exhibiting strong anti-resorptive activity. It effectively inhibits osteoclast differentiation and apoptosis, while also demonstrating anti-cancer properties [1].

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Zoledronic acid disodium tetrahydrate Chemical Structure

Zoledronic acid disodium tetrahydrate, CAS 165800-07-7

Pack Size	Availability	Price/USD
25 mg	6-8 weeks	$ 1,520.00
50 mg	6-8 weeks	$ 1,980.00
100 mg	6-8 weeks	$ 2,500.00

Bulk Inquiry

This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Zoledronic Acid (Zoledronate) disodium tetrahydrate is a potent third-generation bisphosphonate (BP), exhibiting strong anti-resorptive activity. It effectively inhibits osteoclast differentiation and apoptosis, while also demonstrating anti-cancer properties [1].
In vitro	Zoledronic Acid disodium tetrahydrate, at concentrations ranging from 0.1 to 1 μM over 48 hours, enhances receptor activator of nuclear factor kB ligand (RANKL) and sclerostin mRNA expressions in osteocyte-like MLO-Y4 cells, as well as increases osteoclastogenesis supporting factor expression from the same cells. It also amplifies RANKL expression through the IL-6/JAK2/STAT3 pathway in MLO-Y4 cells, while inhibiting osteoclast differentiation and function by modulating NF-κB and JNK signaling pathways. At higher concentrations (10-100 μM, over 1 to 7 days), it significantly reduces the viability of MC3T3-E1 cells and promotes apoptosis, inhibiting cell viability through apoptosis induction at the 4-day mark. This compound also hinders the differentiation and maturation of MC3T3-E1 cells at concentrations below 1 μM. Experiments demonstrated reduced cell viability at 10 μM and 100 μM, with a dose- and time-dependent increase in early apoptotic and late apoptotic or necrotic cells at higher concentrations. Western Blot analysis revealed down-regulation of inactive caspase-3 and increased levels of active caspase-3 at 10 and 100 μM.
In vivo	Zoledronic Acid disodium tetrahydrate administered intraperitoneally (i.p.) at a dose of 0.05 mg/kg weekly for three weeks enhanced bone mineral density and content in five-week-old C57BL6 mice. At increased doses of 0.5-1 mg/kg, administered in the same manner and duration, the compound not only continued to enhance bone mineral density but also inhibited the functions of both osteoclasts and osteoblasts, as well as bone remodeling, thereby affecting the mechanical properties of bone. These findings underline the dose-dependent effects of Zoledronic Acid disodium tetrahydrate on bone health and remodeling.

Molecular Weight	388.113
Formula	C5H16N2Na2O11P2
CAS No.	165800-07-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Zoledronic acid disodium tetrahydrate 165800-07-7 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.