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XTT sodium
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Catalog No. T65679Cas No. 111072-31-2
XTT sodium is a cell viability detector that can be metabolized by mitochondrial dehydrogenase in live cells to produce a highly colored water-soluble formic acid product.
XTT sodium
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Purity: 98.69%
Catalog No. T65679Cas No. 111072-31-2
XTT sodium is a cell viability detector that can be metabolized by mitochondrial dehydrogenase in live cells to produce a highly colored water-soluble formic acid product.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | $40 | - | In Stock | |
| 25 mg | $56 | - | In Stock | |
| 50 mg | $98 | - | In Stock | |
| 100 mg | $179 | - | In Stock | |
| 1 mL x 10 mM (in DMSO) | $44 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Batch Information
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Purity:98.69%
Appearance:Solid
Color:Yellow
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | XTT sodium is a cell viability detector that can be metabolized by mitochondrial dehydrogenase in live cells to produce a highly colored water-soluble formic acid product. |
| In vitro | Assaying Cell Viability Using XTT Sodium [2] 1. Seed cells to be tested into a 96-well plate at 100 μL per well and incubate at 37°C for 40 hours. 2. Dissolve XTT sodium in culture medium to a concentration of 1 mg/mL. Dissolve PMS in phosphate-buffered saline (PBS) to a concentration of 100 mM. Add the PMS solution to the XTT solution to achieve a final PMS concentration of 25 μM. Add 25 μL of the XTT/PMS solution to each well and incubate for 4–8 hours. 3. After incubation, measure absorbance at 450 nm using a microplate reader, with a reference wavelength of 650 nm. Subtract the absorbance value of the blank control. Precautions (1) Prepare XTT solution immediately before use; it may be dissolved in a 60°C water bath. (2) Use PMS solution within one month. (3) Protect PMS and XTT solutions from light during use and storage. (4) Adjust incubation time based on experimental results; prolonged incubation may increase background noise. |
| Molecular Weight | 673.52 |
| Formula | C22H16N7NaO13S2 |
| Cas No. | 111072-31-2 |
| Smiles | O=C(C1=NN([N+](C2=C(OC)C=C([N+]([O-])=O)C(S(=O)([O-])=O)=C2)=N1)C3=C(OC)C=C([N+]([O-])=O)C(S(=O)([O-])=O)=C3)NC4=CC=CC=C4.[Na+] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||
| Solubility Information | H2O: 8 mg/mL (11.88 mM), Sonication is recommended. DMSO: 32 mg/mL (47.51 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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