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Rhodamine B

Name: Rhodamine B
Brand: TargetMol
SKU: T7975
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7975Cas No. 81-88-9

Alias Tetraethylrhodamine, Rhodamine B, Rhodamine O, Brilliant Pink B, Basic Violet 1

Rhodamine B (Brilliant Pink B) is used as a tracer dye in water to determine the rate and direction of flow and transport.

Rhodamine B

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Purity: 97.53%

Catalog No. T7975Alias Tetraethylrhodamine, Rhodamine B, Rhodamine O, Brilliant Pink B, Basic Violet 1Cas No. 81-88-9

Rhodamine B (Brilliant Pink B) is used as a tracer dye in water to determine the rate and direction of flow and transport.

Pack Size	Price	USA Warehouse	Global Warehouse
100 mg	$33	In Stock	In Stock
1 g	$62	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$37	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:97.53%

Appearance:Solid

Color:Yellow to Green

Resource Download

COA HNMR LCMS

Product Introduction

Rhodamine B AI Summary

Rhodamine B exhibits cytotoxicity against various human (518A2, A2780, HT-29, MCF7, A549, 8505C) and mouse NIH/3T3 cell lines, with an EC50 value for cytotoxicity greater than 30,000.0 nM after 96 hours, as determined by the SRB assay, indicating a relatively low level of potency in inducing cell death. Additionally, Rhodamine B shows antiviral activity against Influenza A virus A/WSN/33(H1N1) in MDCK cells, with an IC50 value greater than 100,000.0 nM after 36 hours as determined by the CellTiter-Glo assay, suggesting a weaker antiviral effect. The compound also exhibits bioactivity related to its fluorescence property in DMSO with a quantum yield of 0.5. Furthermore, it demonstrates an apparent permeability in human corneal epithelial cells (HCECs) at a concentration of 0.1%, with a measured apparent permeability value (Papp) of 36.6 x 10^-6 cm/s..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Rhodamine B (Brilliant Pink B) is used as a tracer dye in water to determine the rate and direction of flow and transport.
Synonyms	Tetraethylrhodamine, Rhodamine B, Rhodamine O, Brilliant Pink B, Basic Violet 1

Chemical Properties

Molecular Weight	479.01
Formula	C₂₈H₃₁ClN₂O₃
Cas No.	81-88-9
Smiles	[Cl-].CCN(CC)c1ccc2c(-c3ccccc3C(O)=O)c3ccc(cc3oc2c1)=[N+](CC)CC
Relative Density.	0.79 g/cm3 at 20℃

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 131 mg/mL (273.48 mM), Sonication is recommended.

H2O: 7.14 mg/mL (14.91 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (8.35 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0876 mL	10.4382 mL	20.8764 mL	104.3820 mL
5 mM	0.4175 mL	2.0876 mL	4.1753 mL	20.8764 mL
10 mM	0.2088 mL	1.0438 mL	2.0876 mL	10.4382 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1044 mL	0.5219 mL	1.0438 mL	5.2191 mL
50 mM	0.0418 mL	0.2088 mL	0.4175 mL	2.0876 mL
100 mM	0.0209 mL	0.1044 mL	0.2088 mL	1.0438 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc