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Neoseptin-3

Name: Neoseptin-3
Brand: TargetMol
SKU: T16284
Rating: 4.5 (1 reviews)

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Catalog No. T16284Cas No. 1622863-21-1

Neoseptin-3 is a novel small molecule agonist of mouse mTLR4/MD2 with EC50=18.5 μM that does not activate human TLR4/MD2.

Neoseptin-3

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Catalog No. T16284Cas No. 1622863-21-1

Neoseptin-3 is a novel small molecule agonist of mouse mTLR4/MD2 with EC50=18.5 μM that does not activate human TLR4/MD2.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$99	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Viscous

Color:Transparent

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Product Introduction

Bioactivity

Description	Neoseptin-3 is a novel small molecule agonist of mouse mTLR4/MD2 with EC50=18.5 μM that does not activate human TLR4/MD2.
Targets&IC50	TNF-α induction (RAW264.7 cells):5.9 nM (EC₅₀)
In vitro	Neoseptin-3 is a synthetic small-molecule TLR4-MD-2 agonist that induces strong TNF-α expression in RAW264.7 cells (EC₅₀ = 5.9 ± 0.4 nM), comparable to LPS. Neoseptin-3 also activates TLR4 reporter systems and promotes NF-κB activation[1].
In vivo	In mice, intraperitoneal injection of Neoseptin-3 (2.5 mg/kg) significantly increases serum TNF-α levels, demonstrating in vivo TLR4 agonist activity[1].

Chemical Properties

Molecular Weight	474.59
Formula	C₂₉H₃₄N₂O₄
Cas No.	1622863-21-1
Smiles	C(N[C@@H](CCC1=CC=CC=C1)C(OC(C)(C)C)=O)(=O)C2=CC(CCC3=CC=C(O)C=C3)=C(N)C=C2
Relative Density.	1.174 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (168.57 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.95 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1071 mL	10.5354 mL	21.0708 mL	105.3541 mL
5 mM	0.4214 mL	2.1071 mL	4.2142 mL	21.0708 mL
10 mM	0.2107 mL	1.0535 mL	2.1071 mL	10.5354 mL
20 mM	0.1054 mL	0.5268 mL	1.0535 mL	5.2677 mL
50 mM	0.0421 mL	0.2107 mL	0.4214 mL	2.1071 mL
100 mM	0.0211 mL	0.1054 mL	0.2107 mL	1.0535 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc