Muscimol hydrochloride is the salt form of Muscimol, the main psychoactive component of Amanita muscaria, a potent and selective GABAA receptor agonist and partial GABAC receptor agonist, with no activity on GABAB receptors. It is commonly used in central neuroscience research, with analgesic, anxiolytic and neuroprotective effects, with the advantage of being able to cross the blood-brain barrier and can be taken orally.

Methods: In separate experiments, guinea pigs were pretreated with the selective GABAA antagonist gabazine (3.75 mg/kg，iv) 10 min before control values ​​of the intravenous acetylcholine-induced increase in Ppi were established; Muscimol hydrochloride (187 μg/kg，iv) was then administered 1 min before the subsequent intravenous acetylcholine challenge to investigate the effect of Muscimol hydrochloride on the increase in Ppi induced by intravenous acetylcholine.
Results: Pretreatment with Muscimol hydrochloride (3.75–187 μg/kg) 1 min before the subsequent intravenous acetylcholine challenge significantly reduced the increase in Ppi induced by acetylcholine at each dose tested. [4]