Multitarget AD-IN-3 is a neuroprotective agent capable of crossing the blood-brain barrier. It selectively inhibits MAO-B, with an IC50 of 4.42 μM and a selectivity index (SI) of 18.12. Additionally, it scavenges reactive oxygen species (ROS), inhibits Aβ1-42 self-aggregation, reverses Aβ1-42-induced mitochondrial membrane depolarization, and suppresses apoptosis. Multitarget AD-IN-3 is useful in researching neurological disorders such as Alzheimer's disease.

MAO-B:4.42 μM

Multitarget AD-IN-3 at a concentration of 10 μM significantly inhibits the production of NO, TNF-α, and IL-6 in LPS-stimulated RAW 264.7 and BV-2 cells, while also reducing the expression of COX-2 and NF-κB. It effectively decreases ROS accumulation in TBHP-induced SH-SY5Y cells. Additionally, Multitarget AD-IN-3 can chelate metal ions such as Al 3+, Cu 2+, Fe 2+, and Zn 2+. In concentrations ranging from 25-50 μM over 24 hours, it dose-dependently inhibits the self-aggregation of Aβ 1-42. Furthermore, at 10 μM for 18-24 hours, it reverses Aβ 1-42-induced mitochondrial membrane depolarization in SH-SY5Y cells, inhibits apoptosis, and enhances cell viability.