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ISA-2011B

Name: ISA-2011B
Brand: TargetMol
SKU: T15597
Price: 98 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T15597Cas No. 1395347-24-6

ISA-2011B is an inhibitor of PIP5K1α and can be used in anticancer studies.

ISA-2011B

🥰Excellent

Purity: 99.9%

Catalog No. T15597Cas No. 1395347-24-6

ISA-2011B is an inhibitor of PIP5K1α and can be used in anticancer studies.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$98	In Stock	In Stock
5 mg	$239	In Stock	In Stock
10 mg	$378	In Stock	In Stock
25 mg	$753	In Stock	In Stock
50 mg	$1,130	In Stock	In Stock
100 mg	$1,520	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$263	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Purity:99.9%

ee:98.02%

Appearance:Solid

Color:White

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COA HNMR CSFC LCMS

Product Introduction

Bioactivity

Description	ISA-2011B is an inhibitor of PIP5K1α and can be used in anticancer studies.
In vitro	ISA-2011B significantly decreases AR-V7 and CDK1 levels in both the nucleus and cytoplasm of 22Rv1 cells, demonstrating strong binding affinity towards PIP5K1α and both MARK1 and MARK4 among 460 kinases. In PC-3 cells, ISA-2011B treatment reduces PIP5K1α expression by 78.6%[1] and eliminates nuclear AR expression without affecting cytoplasmic AR[2]. Additionally, ISA-2011B markedly lowers the proliferation rates of PC-3 cells to 58.77%, 48.65%, and 21.62% at doses of 10, 20, and 50 μM, respectively, compared to controls.
In vivo	Overexpression of AR-V7 enhances PIP5K1α levels, accelerating prostate cancer (PCa) growth in xenograft mice. Conversely, the PIP5K1α inhibitor ISA-2011B significantly curtails the proliferation and invasiveness of AR-V7 overexpressing xenograft tumors. ISA-2011B effectively impedes tumor cell growth in xenograft mice by targeting the PIP5K1α-related PI3K/AKT pathways, including those responsible for survival, proliferation, and invasion[1]. Furthermore, ISA-2011B interferes with AR-V7 protein stabilization through its dependency on PIP5K1α, thereby hindering the aggressive growth of AR-V7-high tumors in xenograft models[2].

Chemical Properties

Molecular Weight	423.85
Formula	C₂₂H₁₈ClN₃O₄
Cas No.	1395347-24-6
Smiles	[H][C@@]12Cc3cc4OCOc4cc3[C@H](N1C(=O)CN(C)C2=O)c1c[nH]c2ccc(Cl)cc12
Relative Density.	1.58 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (235.93 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (9.44 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3593 mL	11.7966 mL	23.5933 mL	117.9663 mL
5 mM	0.4719 mL	2.3593 mL	4.7187 mL	23.5933 mL
10 mM	0.2359 mL	1.1797 mL	2.3593 mL	11.7966 mL
20 mM	0.1180 mL	0.5898 mL	1.1797 mL	5.8983 mL
50 mM	0.0472 mL	0.2359 mL	0.4719 mL	2.3593 mL
100 mM	0.0236 mL	0.1180 mL	0.2359 mL	1.1797 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc