GNE-371 is a probe for selective human transcription initiation factor TFIID subunit 1 and transcription initiation factor TFIID subunit 1-like second bromodomain.

TAF12:10 nM

Instructions
1. Solvent selection: GNE-371 is soluble in organic solvents, such as anhydrous dimethyl sulfoxide (DMSO) or acetonitrile. A solution with a concentration of 1–10 mM is usually used, and the specific concentration can be adjusted according to experimental requirements.
2. Labeling reaction: Mix the GNE-371 solution with the molecule to be labeled (such as an antibody or protein). The reaction is usually carried out in a buffer of about pH 8.0, the reaction temperature is generally room temperature, and the time is usually 1–2 hours.
3. Reaction termination and purification: After the reaction is completed, dialysis or gel filtration columns can be used to remove unbound GNE-371 to ensure that the labeled molecules are pure and avoid background signals.
4. Detection and analysis: Use appropriate detection methods, such as fluorescence microscopy, flow cytometry, or fluorescence spectrophotometer to analyze the fluorescence signal of the labeled sample. The excitation wavelength of GNE-371 is 649 nm and the emission wavelength is 667 nm.