GNE-371 is a probe for selective human transcription initiation factor TFIID subunit 1 and transcription initiation factor TFIID subunit 1-like second bromodomain.

TAF12:10 nM

Instructions
1. Solvent selection: GNE-371 is soluble in organic solvents, such as anhydrous dimethyl sulfoxide (DMSO) or acetonitrile. A solution with a concentration of 1–10 mM is usually used, and the specific concentration can be adjusted according to experimental requirements.
2. Labeling reaction: Mix the GNE-371 solution with the molecule to be labeled (such as an antibody or protein). The reaction is usually carried out in a buffer of about pH 8.0, the reaction temperature is generally room temperature, and the time is usually 1–2 hours.
3. Reaction termination and purification: After the reaction is completed, dialysis or gel filtration columns can be used to remove unbound GNE-371 to ensure that the labeled molecules are pure and avoid background signals.
4. Detection and analysis: Use appropriate detection methods, such as fluorescence microscopy, flow cytometry, or fluorescence spectrophotometer to analyze the fluorescence signal of the labeled sample. The excitation wavelength of GNE-371 is 649 nm and the emission wavelength is 667 nm.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

DMSO: 30 mg/mL (69.53 mM), Sonication is recommended.