Glycerophosphocholine

Glycerophosphocholine is a naturally occurring, water-soluble source of choline. As a precursor for the biosynthesis of brain phospholipids, glycerophosphocholine efficiently crosses the blood-brain barrier and increases the availability of acetylcholine in neural tissue. Glycerophosphocholine is used in research on neurodegenerative diseases such as Alzheimer’s disease and dementia. It is also a potential biomarker for breast cancer.

Methods: Caco-2 cells were seeded in Transwell chambers and cultured for 14 days to form a tight monolayer (TEER = 2234 ± 201 Ω·cm²). Different concentrations of Glycerophosphocholine (100 μM, 300 μM, 1 mM) were added to either the apical or basolateral side, After 20 hours of incubation, the apical medium, basal medium, and cell lysates were collected, and the concentrations of Glycerophosphocholine, choline, and betaine were quantified by LC-MS/MS.
Results: Following the addition of Glycerophosphocholine to the apical side, the concentrations of choline on both the apical and basal sides increased significantly in a dose-dependent manner. [1]

Methods: Six-week-old male C57BL/6J mice were administered Glycerophosphocholine (500 mg/kg) via oral gavage. Blood samples were collected from the tail vein at 0, 0.5, 1, 3, 6, and 10 hours post-administration, and the concentrations of TMAO, Glycerophosphocholine, choline, and betaine in the blood were quantified by LC-MS/MS.
Results: Blood concentrations of Glycerophosphocholine and choline increased significantly as early as 0.5 hours post-administration (p < 0.001); TMAO and betaine concentrations peaked between 3 and 6 hours (p < 0.01), confirming that Glycerophosphocholine is rapidly metabolized into choline and subsequently into TMAO. [1]

H2O: 2 mg/mL (7.78 mM), Sonication is recommended.