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CY5-SE Ditriethylamine salt is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Excitation (nm):649, Emission (nm): 670.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 mg | $55 | - | In Stock | |
| 5 mg | $113 | - | In Stock | |
| 10 mg | $167 | - | In Stock | |
| 25 mg | $329 | - | In Stock | |
| 50 mg | $518 | - | In Stock | |
| 100 mg | $749 | - | In Stock |
| Description | CY5-SE Ditriethylamine salt is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Excitation (nm):649, Emission (nm): 670. |
| In vitro | Guidelines (This protocol only provides a guideline, and should be modified according to your specific needs). Conjugation of SELP Analogues with Cy5-SE. 1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice. 2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution. 3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g[1]. |
| Synonyms | CY5-SE Ditriethylamine salt(146368-14-1 Free base) |
| Molecular Weight | 956.26 |
| Formula | C49H73N5O10S2 |
| Smiles | O=C(CCCCCN1C2=CC=C(S(=O)(O)=O)C=C2C(C)(C)C1=CC=CC=CC3=[N+](CC)C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)ON5C(CCC5=O)=O.CCN(CC)CC.CCN(CC)CC |
| Relative Density. | no data available |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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