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Combretastatin A-1

Name: Combretastatin A-1
Brand: TargetMol
SKU: T36848
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T36848Cas No. 109971-63-3

Alias Combretastatin A1

Combretastatin A-1 is a potent microtubule inhibitor with anti-tumour and anti-vascular activity, acting through microtubule protein depolymerisation-mediated inactivation of AKT to inhibit the Wnt/β-catenin pathway, and can be used to study hepatocellular carcinoma.

Combretastatin A-1

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Purity: 97.49%

Catalog No. T36848Alias Combretastatin A1Cas No. 109971-63-3

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$35	-	In Stock
1 mL x 10 mM (in DMSO)	$89	5 days	5 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:97.49%

Appearance:Solid

Color:White

Resource Download

COA HNMR MS HPLC

Product Introduction

Bioactivity

Description	Combretastatin A-1 is a potent microtubule inhibitor with anti-tumour and anti-vascular activity, acting through microtubule protein depolymerisation-mediated inactivation of AKT to inhibit the Wnt/β-catenin pathway, and can be used to study hepatocellular carcinoma.
Targets&IC50	BGC-823 cells:12.2 nM, MDA-MB-231 cells:17.6 nM, NCI-1975 cells:256.3 nM, LM-3:33.8 nM, A375 cells:61 nM, Hepa1-6:32.9 nM, HepG2 cells:9.2 nM, Huh7 cells:728.2 nM, MCF-7 cells:46.0 nM, SMMC-7721 cells:12.8 nM, Bel-7402 cells:38.4 nM, CT-26:1075 nM
In vitro	Combretastatin A-1 (CA1P) induces apoptosis of TAMs in vitro through the same mechanism observed in HepG2 cells, and it eliminates TAMs in the tumor microenvironment (TME) in vivo. Combretastatin A-1 (1-10 nM; 24 hours) induces AKT inactivation and removal of GSK-3β inhibition by microtubule depolymerization, leading to HepG2 cell apoptosis [2]. Combretastatin A-1 (1-50 nM; 6 hours) reduces the mitochondrial membrane potential (MMP) of HepG2 cells and induces the accumulation of ROS in a dose-dependent manner [3].
In vivo	In the HepG2 subcutaneous xenograft model, Combretastatin A-1 (1-4 mg/kg; i.v. every other day for 4 weeks) significantly reduces the tumor volume [3]. In the orthotopic hepatocellular carcinoma mouse model, Combretastatin A-1 (2 mg/kg; every other day for 21 days) demonstrates enhanced apoptosis [3].
Synonyms	Combretastatin A1

Chemical Properties

Molecular Weight	332.35
Formula	C₁₈H₂₀O₆
Cas No.	109971-63-3
Smiles	COc1ccc(\C=C/c2cc(OC)c(OC)c(OC)c2)c(O)c1O

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (240.71 mM), Sonication is recommended.

DMF: 5 mg/mL (15.04 mM), Sonication is recommended.

Ethanol: 3 mg/mL (9.03 mM), Sonication is recommended.

DMSO:PBS (pH 7.2) (1:1): 0.5 mg/mL (1.5 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (9.93 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMF/DMSO

	1mg	5mg	10mg	50mg
5 mM	0.6018 mL	3.0089 mL	6.0178 mL	30.0888 mL

DMF/DMSO

	1mg	5mg	10mg	50mg
10 mM	0.3009 mL	1.5044 mL	3.0089 mL	15.0444 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.1504 mL	0.7522 mL	1.5044 mL	7.5222 mL
50 mM	0.0602 mL	0.3009 mL	0.6018 mL	3.0089 mL
100 mM	0.0301 mL	0.1504 mL	0.3009 mL	1.5044 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc