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Clitocine

Name: Clitocine
Brand: TargetMol
SKU: T30969
Price: 199 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T30969Cas No. 105798-74-1

Clitocine, an adenosine analogue derived from mushrooms, is a potent transcriptional readout agent, an inhibitor of nonsense mutations, and has anticancer activity, inducing the production of p53 protein in cells carrying the p53 nonsense mutation allele.Clitocine enhances TRAIL-mediated apoptosis in human colon cancer cells by promoting Mcl-1 degradation.NSC 81464 is an alkaloid of the yohimbine type extracted from the roots and leaves of Rauwolfia vomitoria.

Clitocine

🥰Excellent

Catalog No. T30969Cas No. 105798-74-1

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$199	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:≥95.0%

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COA HNMR MS

Product Introduction

Bioactivity

Description	Clitocine, an adenosine analogue derived from mushrooms, is a potent transcriptional readout agent, an inhibitor of nonsense mutations, and has anticancer activity, inducing the production of p53 protein in cells carrying the p53 nonsense mutation allele.Clitocine enhances TRAIL-mediated apoptosis in human colon cancer cells by promoting Mcl-1 degradation.NSC 81464 is an alkaloid of the yohimbine type extracted from the roots and leaves of Rauwolfia vomitoria.
In vitro	For premature stop codon readthrough activity, Clitocine incorporation into mRNA is required, and the presence of clitocine at the third position of a premature stop codon is sufficient to promote robust readthrough[2].Clitocine enhances TRAIL lethality in LS411N and SW620 cells, with concentrations ranging from 0 to 0.8 μM over a 24-hour period. Additionally, at a concentration of 0.2 μM for 36 hours, Clitocine significantly potentiates TRAIL-mediated apoptosis[1].
In vivo	Induced by Citocine (0.3-3 mg/kg; s.c.; five times per week), p53 inhibits CAOV-33p53-UAA136 tumor growth in a xenograft model[2].

Chemical Properties

Molecular Weight	287.23
Formula	C₉H₁₃N₅O₆
Cas No.	105798-74-1
Smiles	Nc1ncnc(N[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)c1[N+]([O-])=O
Relative Density.	1.82g/cm3

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (278.52 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (11.49 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4815 mL	17.4077 mL	34.8153 mL	174.0765 mL
5 mM	0.6963 mL	3.4815 mL	6.9631 mL	34.8153 mL
10 mM	0.3482 mL	1.7408 mL	3.4815 mL	17.4077 mL
20 mM	0.1741 mL	0.8704 mL	1.7408 mL	8.7038 mL
50 mM	0.0696 mL	0.3482 mL	0.6963 mL	3.4815 mL
100 mM	0.0348 mL	0.1741 mL	0.3482 mL	1.7408 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc