Shopping Cart

Your shopping cart is currently empty

Clitocine

Name: Clitocine
Brand: TargetMol
SKU: T30969
Price: 199 USD
Availability: InStock
Rating: 5 (10 reviews)

😃Good

Catalog No. T30969Cas No. 105798-74-1

Clitocine, an adenosine analogue derived from mushrooms, is a potent transcriptional readout agent, an inhibitor of nonsense mutations, and has anticancer activity, inducing the production of p53 protein in cells carrying the p53 nonsense mutation allele.Clitocine enhances TRAIL-mediated apoptosis in human colon cancer cells by promoting Mcl-1 degradation.NSC 81464 is an alkaloid of the yohimbine type extracted from the roots and leaves of Rauwolfia vomitoria.

Clitocine

😃Good

Catalog No. T30969Cas No. 105798-74-1

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$199	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:≥95.0%

Appearance:Solid

Color:White

Resource Download

COA HNMR MS

Product Introduction

Bioactivity

Description	Clitocine, an adenosine analogue derived from mushrooms, is a potent transcriptional readout agent, an inhibitor of nonsense mutations, and has anticancer activity, inducing the production of p53 protein in cells carrying the p53 nonsense mutation allele.Clitocine enhances TRAIL-mediated apoptosis in human colon cancer cells by promoting Mcl-1 degradation.NSC 81464 is an alkaloid of the yohimbine type extracted from the roots and leaves of Rauwolfia vomitoria.
In vitro	For premature stop codon readthrough activity, Clitocine incorporation into mRNA is required, and the presence of clitocine at the third position of a premature stop codon is sufficient to promote robust readthrough[2].Clitocine enhances TRAIL lethality in LS411N and SW620 cells, with concentrations ranging from 0 to 0.8 μM over a 24-hour period. Additionally, at a concentration of 0.2 μM for 36 hours, Clitocine significantly potentiates TRAIL-mediated apoptosis[1].
In vivo	Induced by Citocine (0.3-3 mg/kg; s.c.; five times per week), p53 inhibits CAOV-33p53-UAA136 tumor growth in a xenograft model[2].

Chemical Properties

Molecular Weight	287.23
Formula	C₉H₁₃N₅O₆
Cas No.	105798-74-1
Smiles	Nc1ncnc(N[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)c1[N+]([O-])=O
Relative Density.	1.82g/cm3

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (278.52 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (11.49 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4815 mL	17.4077 mL	34.8153 mL	174.0765 mL
5 mM	0.6963 mL	3.4815 mL	6.9631 mL	34.8153 mL
10 mM	0.3482 mL	1.7408 mL	3.4815 mL	17.4077 mL
20 mM	0.1741 mL	0.8704 mL	1.7408 mL	8.7038 mL
50 mM	0.0696 mL	0.3482 mL	0.6963 mL	3.4815 mL
100 mM	0.0348 mL	0.1741 mL	0.3482 mL	1.7408 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc