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Carboxy-PTIO

Catalog No. T38945 CAS 145757-47-7

Carboxy-PTIO is a highly effective nitric oxide (NO) scavenger known for its rapid reaction rate with NO, resulting in the formation of nitric dioxide (NO2). Its direct scavenging action against NO plays a crucial role in preventing hypotension and endotoxic shock, particularly in lipopolysaccharide-stimulated rat models.

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Carboxy-PTIO, CAS 145757-47-7

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Description	Carboxy-PTIO is a highly effective nitric oxide (NO) scavenger known for its rapid reaction rate with NO, resulting in the formation of nitric dioxide (NO2). Its direct scavenging action against NO plays a crucial role in preventing hypotension and endotoxic shock, particularly in lipopolysaccharide-stimulated rat models.
In vitro	Carboxy-PTIO, at a concentration of 200 μM and administered 1 hour before physalin A for a duration of 24 hours, effectively inhibits the increase in NO expression triggered by physalin A, without affecting baseline NO levels[1]. Furthermore, it mitigates the physalin A-induced activation of apoptotic pathways by preventing the cleavage of procaspase-3 and PARP, downregulating ICAD expression, and reducing nuclear DNA fragmentation[1]. Additionally, Carboxy-PTIO does not alter iNOS expression levels but negates the physalin A-induced reduction in both mTOR and phosphorylated mTOR, while also inhibiting the autophagic process by preventing the conversion of LC3 I to LC3 II in A375-S2 cells[1]. This was corroborated by a Cell Viability Assay on A375-S2 cells, where Carboxy-PTIO, pre-administered at 200 μM for 1 hour followed by physalin A exposure for 24 hours, decreased the cleavage of procaspase-3 and PARP induced by physalin A[1].
In vivo	Carboxy-PTIO (intravenous injection; 0.056-1.70 mg/kg/min; infused for 1 hr beginning 90 min after the LPS injection) has been demonstrated to significantly ameliorate hypotension, renal dysfunction, and enhance survival rates in LPS-treated rats, showcasing its potent therapeutic efficacy in endotoxin shock by directly scavenging nitric oxide (NO). However, it does not influence these parameters in normal rats. The study utilized SD rats as the animal model, with a dosage range of 0.056-1.70 mg/kg/min administered intravenously.

Molecular Weight	277.3
Formula	C14H17N2O4
CAS No.	145757-47-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Hao He, et al.Nitric oxide induces apoptosis and autophagy; autophagy down-regulates NO synthesis in physalin A-treated A375-S2 human melanoma cells.Food Chem Toxicol. 2014 Sep;71:128-35. 2. T Akaike, et al. Antagonistic action of imidazolineoxyl N-oxides against endothelium-derived relaxing factor/.NO through a radical reaction. Biochemistry. 1993 Jan 26;32(3):827-32. 3. M Yoshid, et al. Therapeutic effects of imidazolineoxyl N-oxide against endotoxin shock through its direct nitric oxide-scavenging activity. Biochem Biophys Res Commun. 1994 Jul 29;202(2):923-30.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Carboxy-PTIO 145757-47-7 CarboxyPTIO Carboxy PTIO inhibitor inhibit

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