Bryostatin 1

Name: Bryostatin 1
Brand: TargetMol
SKU: T14831
Rating: 4.5 (1 reviews)

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Catalog No. T14831Cas No. 83314-01-6

Bryostatin 1 is a natural macrolide isolated from the bryozoan Bugula neritina and is a CNS-permeable PKC modulator. Bryostatin 1 binds to the isolated C1 domain of Munc13-1 and the full-length Munc13-1 protein [Kis: 8.07 nM and 0.45 nM].

Bryostatin 1

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Catalog No. T14831Cas No. 83314-01-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 μg	$859	35 days	35 days

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Product Introduction

Bioactivity

Description	Bryostatin 1 is a natural macrolide isolated from the bryozoan Bugula neritina and is a CNS-permeable PKC modulator. Bryostatin 1 binds to the isolated C1 domain of Munc13-1 and the full-length Munc13-1 protein [Kis: 8.07 nM and 0.45 nM].
In vitro	Bryostatin 1 (1 μM; 5 minutes; HT22 cells) treatment successfully recruits Munc13-1 from the cytosol to the plasma membrane. Effects of Bryostatin 1 on the other Munc13 family members, ubMunc13-2 and bMunc13-2, resembled those of Munc13-1 for translocation. The increased level of expression of Munc13-1 following a 24 h incubation with Bryostatin 1 in both HT22 and primary mouse hippocampal cells is observed [1]. Bryostatin 1 can also affect the immune system by modulating dendritic cells (DCs) via TLR4 through the MyD88-independent pathway which favors an anti-inflammatory environment by inducing a type 2 phenotype that promotes the differentiation of CD4+ T-helper (Th) lymphocytes into Th2 versus Th1 effector cells [2].
In vivo	Bryostatin 1 (30 μg/kg; i.p.; 3 days per week; for 2 weeks) treatment completely prevents the onset of EAE [2].

Chemical Properties

Molecular Weight	905.03
Formula	C₄₇H₆₈O₁₇
Cas No.	83314-01-6
Smiles	O[C@]12[C@@H](OC(/C=C/C=C/CCC)=O)\C(=C\C(OC)=O)\C[C@](O1)(C[C@]([C@@H](C)O)(OC(=O)C[C@H](O)C[C@]3(O[C@@](O)(C[C@]4(O[C@@](\C=C\C2(C)C)(C/C(=C\C(OC)=O)/C4)[H])[H])C(C)(C)[C@@H](OC(C)=O)C3)[H])[H])[H]
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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