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Bicyclomycin benzoate

Name: Bicyclomycin benzoate
Brand: TargetMol
SKU: T10541
Rating: 4.5 (1 reviews)

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Catalog No. T10541Cas No. 37134-40-0

Alias FR2054, FR 2054

Bicyclomycin benzoate, an antibiotic, exhibits activity against a broad spectrum of Gram-negative/Gram-positive bacteria.

Bicyclomycin benzoate

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🥰Excellent

Catalog No. T10541Alias FR2054, FR 2054Cas No. 37134-40-0

Bicyclomycin benzoate, an antibiotic, exhibits activity against a broad spectrum of Gram-negative/Gram-positive bacteria.

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Product Introduction

Bioactivity

Description	Bicyclomycin benzoate, an antibiotic, exhibits activity against a broad spectrum of Gram-negative/Gram-positive bacteria.
In vitro	Bicyclomycin interferes with the biosynthesis of lipoprotein and its assembly to peptidoglycan in the cell envelope of E. coli. At the lethal level, Bicyclomycin is shown to inhibit the synthesis of RNA and protein in the growing cells of E. coli 15 THU [1]. Bicyclomycin targets the rho transcription termination factor in Escherichia coli. Bicyclomycin can disrupt the rho molecular machinery thereby leading to a catastrophic effect caused by the untimely overproduction of proteins not normally expressed constitutively, thus leading to a toxic effect on the cells [2]. The inhibition of rho poly(C)-stimulated hydrolysis of ATP by Bicyclomycin has been found to proceed by a non-competitive, reversible pathway with respect to ATP (Ki: 20 μM) [3].
In vivo	Following a single intramuscular administration of Bicyclomycin at a dosage of 50 mg/kg in rats, the compound exhibits a low rate of excretion. It is well distributed across different tissues, with the peak concentration recorded in the kidneys at a level of 100 mg/kg [4].
Synonyms	FR2054, FR 2054

Chemical Properties

Molecular Weight	406.39
Formula	C₁₉H₂₂N₂O₈
Cas No.	37134-40-0
Smiles	C[C@](O)(COC(=O)c1ccccc1)[C@H](O)[C@@]12NC(=O)[C@@](O)(NC1=O)C(=C)CCO2
Relative Density.	1.49 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (196.86 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (8.12 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4607 mL	12.3035 mL	24.6069 mL	123.0345 mL
5 mM	0.4921 mL	2.4607 mL	4.9214 mL	24.6069 mL
10 mM	0.2461 mL	1.2303 mL	2.4607 mL	12.3035 mL
20 mM	0.1230 mL	0.6152 mL	1.2303 mL	6.1517 mL
50 mM	0.0492 mL	0.2461 mL	0.4921 mL	2.4607 mL
100 mM	0.0246 mL	0.1230 mL	0.2461 mL	1.2303 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc