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ASC-69

Name: ASC-69
Brand: TargetMol
SKU: T82960
Rating: 4.5 (1 reviews)

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Catalog No. T82960Cas No. 1216665-50-7

Alias ASC69, APY-69, APY69

ASC-69 (APY69) is a potent small-molecule PD-1/PD-L1 signaling pathway inhibitor that restores T-cell immune function by blocking the interaction between PD-1 and PD-L1. ASC-69 is widely used in both in vitro and in vivo experimental models related to tumor immunology, immune checkpoint regulatory mechanism research, and anti-tumor drug development to evaluate its immune-enhancing and anti-cancer potential.

ASC-69

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Catalog No. T82960Alias ASC69, APY-69, APY69Cas No. 1216665-50-7

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 mg	Inquiry	8-10 weeks	8-10 weeks
50 mg	Inquiry	8-10 weeks	8-10 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	ASC-69 (APY69) is a potent small-molecule PD-1/PD-L1 signaling pathway inhibitor that restores T-cell immune function by blocking the interaction between PD-1 and PD-L1. ASC-69 is widely used in both in vitro and in vivo experimental models related to tumor immunology, immune checkpoint regulatory mechanism research, and anti-tumor drug development to evaluate its immune-enhancing and anti-cancer potential.
Targets&IC50	PD-L1:1.86 nM
In vitro	In HTRF assays, ASC-69 inhibited the PD-1/PD-L1 interaction with an IC50 of 1.86 nM by inducing PD-L1 dimerization [1].
In vivo	In syngeneic mouse models, including CT26 and MC38, oral or intraperitoneal (i.p.) administration of ASC-69 results in dose-dependent tumor growth inhibition. The antitumor efficacy observed is comparable to that of anti-PD-L1 monoclonal antibodies. The presence of the difluoromethyleneoxy linker contributes to the compound's metabolic stability and oral bioavailability in rodent models
Synonyms	ASC69, APY-69, APY69

Chemical Properties

Molecular Weight	345.4
Formula	C₁₉H₁₉N₇
Cas No.	1216665-50-7
Smiles	N#CCC1=CC=C(C=C1)NC=2N=C(C=C(N2)C)NC3=NNC(=C3)C4CC4

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (231.62 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8952 mL	14.4760 mL	28.9519 mL	144.7597 mL
5 mM	0.5790 mL	2.8952 mL	5.7904 mL	28.9519 mL
10 mM	0.2895 mL	1.4476 mL	2.8952 mL	14.4760 mL
20 mM	0.1448 mL	0.7238 mL	1.4476 mL	7.2380 mL
50 mM	0.0579 mL	0.2895 mL	0.5790 mL	2.8952 mL
100 mM	0.0290 mL	0.1448 mL	0.2895 mL	1.4476 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc