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ASC-69

Name: ASC-69
Brand: TargetMol
SKU: T82960
Rating: 4.5 (1 reviews)

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Catalog No. T82960Cas No. 1216665-50-7

Alias ASC69, APY-69, APY69

ASC-69 (APY69) is a potent small-molecule PD-1/PD-L1 signaling pathway inhibitor that restores T-cell immune function by blocking the interaction between PD-1 and PD-L1. ASC-69 is widely used in both in vitro and in vivo experimental models related to tumor immunology, immune checkpoint regulatory mechanism research, and anti-tumor drug development to evaluate its immune-enhancing and anti-cancer potential.

ASC-69

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Catalog No. T82960Alias ASC69, APY-69, APY69Cas No. 1216665-50-7

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 mg	Inquiry	8-10 weeks	8-10 weeks
50 mg	Inquiry	8-10 weeks	8-10 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

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Product Introduction

Bioactivity

Description	ASC-69 (APY69) is a potent small-molecule PD-1/PD-L1 signaling pathway inhibitor that restores T-cell immune function by blocking the interaction between PD-1 and PD-L1. ASC-69 is widely used in both in vitro and in vivo experimental models related to tumor immunology, immune checkpoint regulatory mechanism research, and anti-tumor drug development to evaluate its immune-enhancing and anti-cancer potential.
Targets&IC50	PD-L1:1.86 nM
In vitro	In HTRF assays, ASC-69 inhibited the PD-1/PD-L1 interaction with an IC50 of 1.86 nM by inducing PD-L1 dimerization [1].
In vivo	In syngeneic mouse models, including CT26 and MC38, oral or intraperitoneal (i.p.) administration of ASC-69 results in dose-dependent tumor growth inhibition. The antitumor efficacy observed is comparable to that of anti-PD-L1 monoclonal antibodies. The presence of the difluoromethyleneoxy linker contributes to the compound's metabolic stability and oral bioavailability in rodent models
Synonyms	ASC69, APY-69, APY69

Chemical Properties

Molecular Weight	345.4
Formula	C₁₉H₁₉N₇
Cas No.	1216665-50-7
Smiles	N#CCC1=CC=C(C=C1)NC=2N=C(C=C(N2)C)NC3=NNC(=C3)C4CC4

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (231.62 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8952 mL	14.4760 mL	28.9519 mL	144.7597 mL
5 mM	0.5790 mL	2.8952 mL	5.7904 mL	28.9519 mL
10 mM	0.2895 mL	1.4476 mL	2.8952 mL	14.4760 mL
20 mM	0.1448 mL	0.7238 mL	1.4476 mL	7.2380 mL
50 mM	0.0579 mL	0.2895 mL	0.5790 mL	2.8952 mL
100 mM	0.0290 mL	0.1448 mL	0.2895 mL	1.4476 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline /PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Stock solution preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(stock solution concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline /PBS/ddH₂O TargetMol | reagent

mix well and clarify

The above example illustrates how to use "In Vivo Formulation Calculator" and does not represent a recommended formulation for any specific compound. Please select an appropriate dissolution method based on your experimental animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc