Apoptosis inducer53 is an apoptosis inducer that inhibits the proliferation of human tumor cell lines (A549, HeLa, SW1573, T-47D, WiDr) with a GI50 value of 2.5-9.1 μM. It induces apoptosis in cancer cells and decreases colony formation. Furthermore, Apoptosis inducer53 activates the p38α MAPK signaling pathway and exhibits anti-inflammatory effects. It also shows activity against the protozoan parasite Leishmania donovani. Apoptosis inducer53 is applicable in research related to cancer, infections, and inflammation.

Apoptosis inducer 53 (Compound 24) effectively inhibits the proliferation of various human tumor cell lines, including A549, HeLa, SW1573, T-47D, and WiDr, with GI50 values of 3.7 μM, 2.5 μM, 3.7 μM, 9.1 μM, and 4.9 μM respectively. At a concentration of 25 μM for 20 hours, it induces typical apoptotic phenotypes in HeLa cells. It also reduces HeLa cell colony formation in a dose-dependent manner at concentrations of 7.5-12.5 μM over 24 hours or 0.83-2.5 μM over 7 days. Furthermore, Apoptosis inducer 53 inhibits the growth of Leishmania donovani amastigotes in THP-1 differentiated macrophages, with an IC50 of 2.59 μM. Additionally, at 10 μM concentration for 24 hours, it suppresses the production of NLRP3, Caspase-1, and IL-1β in LPS-stimulated BV.2 microglial cells by activating p38α MAPK signaling.