Antitumor agent-143 (compound 2c) is an antitumor agent that inhibits cell proliferation during the S phase in A549 cells and induces early apoptosis (apoptosis). It leads to cell death via ferroptosis (ferroptosis), apoptosis through ROS-mediated mitochondrial dysfunction, and pyroptosis (pyroptosis) mediated by GSDMD.

Antitumor agent-143 (compound 2c; 48h) inhibits the growth of A549, B16, HCT116, and HepG2 cells, with IC50 values of 2.2 μM, 2.5 μM, 2.5 μM, and 1 μM, respectively. It halts cell proliferation in the S phase and triggers early apoptosis in A549 cells. The agent increases ROS and Ca2+ levels, leading to reduced mitochondrial membrane potential, opening of the mitochondrial permeability transition pore, and increased cytochrome c.

Compound 2c, known as Antitumor agent-143, was observed to reduce tumor volume in mice.