Anticancer agent 194 (compound 10p), a ferroptosis and autophagy inducer, arrests the colon cancer cell cycle at the G2/M phase without inducing apoptosis, and independently triggers cell ferroptosis and autophagy through the massive accumulation of ROS [1].

Anticancer Agent 194 (Compound 10p; 1-10 μM; 48 hours) exhibits anti-proliferative activity against HT-29 cancer cells with an IC50 of 1.97 μM[1]. At concentrations of 1-4 μM, this compound arrests the cell cycle in the G2/M phase and decreases the expression of GPX4 in a concentration-dependent manner[1]. Moreover, Anticancer Agent 194 induces autophagy in HT-29 cells within a concentration range of 0.5-4 μM[1]. Cell viability assays indicate an increase in cell death after treatment for 48 hours at concentrations of 1 μM, 5 μM, and 10 μM. Cell cycle analysis reveals arrest at the G2/M phase at concentrations of 1 μM, 2 μM, and 4 μM. Western Blot analysis shows a decrease in GPX4 expression as concentration increases. Autophagy assays demonstrate the occurrence of autophagy in HT-29 cells at concentrations of 1 μM, 2 μM, and 4 μM[1].