ALV2

Name: ALV2
Brand: TargetMol
SKU: T40099
Rating: 4.5 (1 reviews)

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Catalog No. T40099Cas No. 2438124-95-7

ALV2 is a potent and selective Helios degrader that binds to CRBN protein, with an IC50 of 0.57 μM. Helios, a zinc-finger transcription factor, is essential for maintaining a stable T reg cell phenotype in the inflammatory tumor microenvironment.

ALV2

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Catalog No. T40099Cas No. 2438124-95-7

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Product Introduction

Bioactivity

Description	ALV2 is a potent and selective Helios degrader that binds to CRBN protein, with an IC50 of 0.57 μM. Helios, a zinc-finger transcription factor, is essential for maintaining a stable T reg cell phenotype in the inflammatory tumor microenvironment.
Targets&IC50	CRBN:0.57 μM (IC50)
In vitro	ALV2, within a concentration range of 10 nM to 100 μM, initiates CRBN-Helios dimerization as evidenced by the TR-FRET assay[1]. At lower concentrations (0.1-10 μM), ALV2 specifically facilitates the degradation of Helios in Jurkat cells[1]. Moreover, when pre-administered for 18 hours at a concentration of 1 μM, ALV2 enhances IL-2 secretion in Jurkat cells[1].
In vivo	ALV2, administered intraperitoneally at a dosage of 100 mg/kg twice daily for 7 days, selectively induces Helios degradation in vivo in Crbn I391V/I391V mouse models. This treatment specifically reduced Helios levels without affecting Ikaros in splenic CD4 + FoxP3 + T regulatory cells.

Chemical Properties

Molecular Weight	523.97
Formula	C₂₆H₂₆ClN₅O₅
Cas No.	2438124-95-7
Smiles	Cc1ccc(NC(=O)NC(C)(C)c2cccc(NC3=CC(=O)N(C4CCC(=O)NC4=O)C3=O)c2)cc1Cl

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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