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Ac-IEPD-AFC

Name: Ac-IEPD-AFC
Brand: TargetMol
SKU: TP1128
Price: 44 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. TP1128Cas No. 1135417-31-0

Ac-IEPD-AFC (IEPD) is a fluorescent substrate for granzyme B and can be used to measure granzyme B activity.

Ac-IEPD-AFC

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Purity: 99.16%

Catalog No. TP1128Cas No. 1135417-31-0

Ac-IEPD-AFC (IEPD) is a fluorescent substrate for granzyme B and can be used to measure granzyme B activity.

Pack Size	Price	Availability
1 mg	$44	In Stock
5 mg	$89	In Stock
10 mg	$147	In Stock
25 mg	$248	In Stock
50 mg	$368	In Stock
100 mg	$543	In Stock

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Batch Information

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Purity:99.16%

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COA HPLC MS

Product Introduction

Bioactivity

Description

Ac-IEPD-AFC (IEPD) is a fluorescent substrate for granzyme B and can be used to measure granzyme B activity.

Chemical Properties

Molecular Weight	725.67
Formula	C₃₂H₃₈F₃N₅O₁₁
Cas No.	1135417-31-0
Smiles	C(F)(F)(F)C=1C=2C(=CC(NC([C@@H](NC(=O)[C@H]3N(C([C@@H](NC([C@H]([C@H](CC)C)NC(C)=O)=O)CCC(O)=O)=O)CCC3)CC(O)=O)=O)=CC2)OC(=O)C1
Relative Density.	1.438 g/cm3 (Predicted)
Color	White
Appearance	Solid
Sequence	Ac-IEPD-AFC
Sequence Short	XEPD

Storage & Solubility Information

Storage

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (110.24 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.3780 mL	6.8902 mL	13.7804 mL	68.9018 mL
5 mM	0.2756 mL	1.3780 mL	2.7561 mL	13.7804 mL
10 mM	0.1378 mL	0.6890 mL	1.3780 mL	6.8902 mL
20 mM	0.0689 mL	0.3445 mL	0.6890 mL	3.4451 mL
50 mM	0.0276 mL	0.1378 mL	0.2756 mL	1.3780 mL
100 mM	0.0138 mL	0.0689 mL	0.1378 mL	0.6890 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc