[12]-Dehydrogingerdione is a natural product found in ginger that has anti-neuroinflammatory activity, possibly by inhibiting the Akt/IKK/IκB/NF-κB pathway and promoting the Nrf-2/HO-1 pathway to prevent neuroinflammation. 12-Dehydrogingerdione can inhibit the production of NO, IL-6, and PGE2 in LPS-stimulated Raw 264.7 cells.

METHODS: Microglia were treated with [12]-Dehydrogingerdione (2.5, 5, 10 μM, 12 hours) in the presence or absence of LPS. Cell viability was measured before and after treatment. ELISA kits were used to detect the secretion of proinflammatory cytokines TNF-α and IL-6 in LPS-activated microglia.
RESULTS [12]-Dehydrogingerdione (10 μM) had no significant effect on microglial viability in the presence or absence of LPS, while high concentrations of [12]-Dehydrogingerdione significantly induced cytotoxicity; treatment with 12-DHGD dose-dependently inhibited LPS-induced TNFα and IL-6 secretion. [1]
METHODS: [12]-Dehydrogingerdione (2.5, 5, 10 μM) was used to treat microglia, and its effect on the transcription factor NF-κB in LPS-activated BV-2 microglia was studied.
RESULTS [12]-Dehydrogingerdione treatment not only inhibited the phosphorylation and translocation of NF-κB, but also downregulated the DNA binding ability of NF-κB. [1]