Powder: -20°C for 3 years
In solvent: -80°C for 2 years
MLN0905 is an effective PLK1 inhibitor(IC50=2 nM).
Description | MLN0905 is an effective PLK1 inhibitor(IC50=2 nM). |
Targets&IC50 | PLK1:2 nM |
Kinase Assay | PLK1 Flash Plate Assay: The human PLK1 enzymatic reaction totaling 30μL contained 50 mM Tris-HCl (pH 8.0), 10 mM MgCl2, 0.02% BSA, 10% glycerol, 1 mM DTT, 100 mM NaCl, 3.3% DMSO, 8 μM ATP, 0.2μCi [γ- 33 P]-ATP, 4 μM peptide substrate (Biotin-AHX-LDETGHLDSSGLQEVHLA-CONH2), and 10 nM recombinant human PLK1[2–369]T210D. The enzymatic reaction mixture, with or without PLK inhibitors, is incubated for 2.5 h at 30℃before termination with 20μL of 150 mM EDTA. Then 25μL of the stopped enzyme reaction mixture is transferred to a 384-well streptavidin coated Image FlashPlate and incubated at room temperature for 3 h. The Image Flash Plate wells are washed three times with 0.02% Tween-20 and then read on the Perkin-Elmer Viewlux. |
Synonyms | PLK1 Inhibitor |
Molecular Weight | 486.56 |
Formula | C24H25F3N6S |
CAS No. | 1228960-69-7 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
Ethanol: <1 mg/mL
DMSO: 90 mg/mL (185 mM)
H2O: <1 mg/mL
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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MLN0905 1228960-69-7 Cell Cycle/Checkpoint PLK inhibit Inhibitor Polo-like Kinase (PLK) PLK1 Inhibitor MLN-0905 MLN 0905 inhibitor