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ML239

Catalog No. T4399 CAS 1378872-36-6

Synonyms: CID-49843203

ML239 (CID-49843203) is a potent and selective inhibitor of breast cancer stem cells, with an IC50 of 1.16 μM.

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ML239, CAS 1378872-36-6

Pack Size	Availability	Price/USD
5 mg	In stock	$ 43.00
10 mg	In stock	$ 72.00
25 mg	In stock	$ 143.00
50 mg	In stock	$ 278.00
100 mg	In stock	$ 477.00
1 mL * 10 mM (in DMSO)	In stock	$ 48.00

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Purity: 99.82%

Biological Description

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Storage & Solubility Information

Description	ML239 (CID-49843203) is a potent and selective inhibitor of breast cancer stem cells, with an IC50 of 1.16 μM.
Targets&IC50	Breast cancer stem cells:1.16 μM.
In vitro	ML239 (Compound 7j) serves as a powerful and selective inhibitor targeting breast cancer stem cells, exhibiting an IC50 value of 1.16 μM and demonstrating approximately 24-fold greater selectivity compared to the control cell line. It operates primarily by potentially activating fatty acid desaturase 2 (FADS2), thereby inhibiting the growth of breast cancer stem-like cells. Additionally, ML239 shows cytotoxic effects on NCIH661 cells. Interestingly, the reduction of FADS2 activity, either through direct knockdown or by using the FADS2 inhibitor SC-26196, diminishes the cytotoxicity of ML239 in cancer cell lines (CCLs), suggesting a critical role of FADS2 in ML239's mechanism of action.
Kinase Assay	ML239 is dissolved in DMSO.Cancer cell lines (CCLs) are plated at a density of 500 cells/well in white opaque tissue-culture-treated Aurora 1536-well MaKO plates in the provider-recommended growth media using a highly automated platform. Compounds (ML239) are added by acoustic transfer using a Labcyte Echo 555. 24 hours after plating. The effects of small molecules (ML239) are measured over a 16-point concentration range (two-fold dilution) in duplicate. DMSO is used at a constant concentration of 0.33%, including vehicle-only control wells. As a surrogate for viability, cellular ATP levels are assessed 72 hours after compound transfer by addition of CellTiterGlo followed by luminescence measurement using a ViewLux Microplate Imager. Duplicates are averaged and luminescence values normalized to vehicle (DMSO) treatment and background (media-only) wells

Synonyms	CID-49843203
Molecular Weight	346.6
Formula	C13H10Cl3N3O2
CAS No.	1378872-36-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 300 mg/mL

Ethanol: 50 mM

References and Literature

1. Germain AR,etal.Identification of a selective small molecule inhibitor of breast cancer stem cells.Bioorg Med Chem Lett. 2012 May 15;22(10):3571-4. 2. Rees MG,etal.Correlating chemical sensitivity and basal gene expression reveals mechanism of action.Nat Chem Biol. 2016 Feb;12(2):109-16.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Anti-Cancer Compound Library Bioactive Compounds Library Max NO PAINS Compound Library Bioactive Compound Library Anti-Breast Cancer Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

ML239 1378872-36-6 Others ML 239 inhibit Inhibitor CID-49843203 CID49843203 CID 49843203 ML-239 inhibitor

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