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5-Ph-IAA

Name: 5-Ph-IAA
Brand: TargetMol
SKU: T8885
Price: 58 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T8885 Copy Product Info

Purity: 99.97%

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5-Ph-IAA is an IAA derivative and a highly efficient, low-toxicity, reversible protein degradation ligand specifically designed for the AID2 system. AID2 induces rapid and effective depletion of mAID fusion proteins, enabling the study of protein function in living cells; it also exhibits antitumor activity. 5-Ph-IAA can be used in antitumor research.

Cas No. 168649-23-8

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Pack Size	Price	USA Stock	Global Stock
5 mg	$58	In Stock	In Stock
10 mg	$97	In Stock	In Stock
25 mg	$197	In Stock	In Stock
50 mg	$329	In Stock	In Stock
100 mg	$558	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$64	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.97%

Appearance:Solid

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COA HNMR LCMS

Product Introduction

5-Ph-IAA AI Summary

5-Ph-IAA has a rate constant for oxidation by HRP of 46,000 M^-1 s^-1. Moreover, it exhibits no cytotoxicity against Chinese hamster lung fibroblasts, with a surviving fraction of 1.0 determined through a clonogenic assay..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	5-Ph-IAA is an IAA derivative and a highly efficient, low-toxicity, reversible protein degradation ligand specifically designed for the AID2 system. AID2 induces rapid and effective depletion of mAID fusion proteins, enabling the study of protein function in living cells; it also exhibits antitumor activity. 5-Ph-IAA can be used in antitumor research.
In vitro	Methods: HCT116 cells (human colorectal cancer cell line) were treated with IAA (100 μM) or 5-Ph-IAA (1 μM) for 24 and 72 hours, followed by detection of cell cycle changes. Results: Treatment with 1 μM 5-Ph-IAA had no significant effect on the cell cycle. [1]
In vivo	Methods: CRISPR/Cas9 was used to knock in an AID tag downstream of the start codon of the endogenous Dcp2 gene, generating AID-Dcp2 mice. This strain was crossed with a systemically TIR1-expressing line. Embryos were collected 20 hours after intraperitoneal injection of 5 mg/kg 5-Ph-IAA. DCP2 protein levels were detected by Western blot, and phenotypes were observed. Results: Western Blot analysis revealed near-complete disappearance of DCP2 protein following 5-Ph-IAA treatment. Within 20 hours post-treatment, embryos exhibited phenotypes similar to Dcp2 knockout mice, demonstrating significantly accelerated protein degradation compared to conventional knockout Methods.[2]

Chemical Properties

Molecular Weight	251.28
Formula	C₁₆H₁₃NO₂
Cas No.	168649-23-8
Smiles	OC(=O)Cc1c[nH]c2ccc(cc12)-c1ccccc1
Relative Density.	1.291 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (179.08 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4.5 mg/mL (17.91 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.9796 mL	19.8981 mL	39.7962 mL	198.9812 mL
5 mM	0.7959 mL	3.9796 mL	7.9592 mL	39.7962 mL
10 mM	0.3980 mL	1.9898 mL	3.9796 mL	19.8981 mL
20 mM	0.1990 mL	0.9949 mL	1.9898 mL	9.9491 mL
50 mM	0.0796 mL	0.3980 mL	0.7959 mL	3.9796 mL
100 mM	0.0398 mL	0.1990 mL	0.3980 mL	1.9898 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.